Anti-SIAH1 antibody [EPR27101-45] - BSA and Azide free,Abcam,AB305250
Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies . Conjugation ready Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold. Compatibility This product is compatible with the Maxpar ® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar ® is a trademark of Fluidigm Canada Inc.
Host
Rabbit
Reactivity
Human
Application
WB
Platform ID
BAB720843238
Abcam
Contact
Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215
Email:
Specifications
Scientific Background
Target data E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins (PubMed : 14506261, PubMed : 14645235, PubMed : 14654780, PubMed : 15064394, PubMed : 16085652, PubMed : 19224863, PubMed : 20508617, PubMed : 22483617, PubMed : 28546513, PubMed : 32430360, PubMed : 33591310, PubMed : 9334332, PubMed : 9858595). E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates (PubMed : 14506261, PubMed : 14645235, PubMed : 14654780, PubMed : 15064394, PubMed : 16085652, PubMed : 19224863, PubMed : 20508617, PubMed : 22483617, PubMed : 9334332, PubMed : 9858595). Mediates E3 ubiquitin ligase activity either through direct binding to substrates or by functioning as the essential RING domain subunit of larger E3 complexes (PubMed : 14506261, PubMed : 14645235, PubMed : 14654780, PubMed : 15064394, PubMed : 16085652, PubMed : 19224863, PubMed : 20508617, PubMed : 22483617, PubMed : 9334332, PubMed : 9858595). Triggers the ubiquitin-mediated degradation of many substrates, including proteins involved in transcription regulation (ELL2, MYB, POU2AF1, PML and RBBP8), a cell surface receptor (DCC), the cell-surface receptor-type tyrosine kinase FLT3, the cytoplasmic signal transduction molecules (KLF10/TIEG1 and NUMB), an antiapoptotic protein (BAG1), a microtubule motor protein (KIF22), a protein involved in synaptic vesicle function in neurons (SYP), a structural protein (CTNNB1) and SNCAIP (PubMed : 10747903, PubMed : 11146551, PubMed : 11389839, PubMed : 11389840, PubMed : 11483517, PubMed : 11483518, PubMed : 11752454, PubMed : 12072443). Confers constitutive instability to HIPK2 through proteasomal degradation (PubMed : 18536714, PubMed : 33591310). It is thereby involved in many cellular processes such as apoptosis, tumor suppression, cell cycle, axon guidance, transcription regulation, spermatogenesis and TNF-alpha signaling (PubMed : 14506261, PubMed : 14645235, PubMed : 14654780, PubMed : 15064394, PubMed : 16085652, PubMed : 19224863, PubMed : 20508617, PubMed : 22483617, PubMed : 9334332, PubMed : 9858595). Has some overlapping function with SIAH2 (PubMed : 14506261, PubMed : 14645235, PubMed : 14654780, PubMed : 15064394, PubMed : 16085652, PubMed : 19224863, PubMed : 20508617, PubMed : 22483617, PubMed : 9334332, PubMed : 9858595). Induces apoptosis in cooperation with PEG3 (By similarity). Upon nitric oxid (NO) generation that follows apoptotic stimulation, interacts with S-nitrosylated GAPDH, mediating the translocation of GAPDH to the nucleus (By similarity). GAPDH acts as a stabilizer of SIAH1, facilitating the degradation of nuclear proteins (By similarity). Mediates ubiquitination and degradation of EGLN2 and EGLN3 in response to the unfolded protein response (UPR), leading to their degradation and subsequent stabilization of ATF4 (By similarity). Also part of the Wnt signaling pathway in which it mediates the Wnt-induced ubiquitin-mediated proteasomal degradation of AXIN1 (PubMed : 28546513, PubMed : 32430360). See full target information SIAH1
Category Paths
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