Anti-SQSTM1 / p62 antibody [EPR18351] - BSA and Azide free,Abcam,AB227992
What is this antibody validated in? Anti-SQSTM1 / p62 antibody [EPR18351] - BSA and Azide free (ab227992) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples. What is the molecular weight of SQSTM1 / p62? Anti-SQSTM1 / p62 [EPR18351] - BSA and Azide free (ab227992) specifically detects a band for SQSTM1 / p62 (UniProt: Q13501) at a molecular weight of 47kDa. Trial sizes available! Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies. Specificity confirmed The specificity of Anti-SQSTM1 / p62 antibody [EPR18351] - BSA and Azide free (ab227992) has been confirmed by Immunocytochemistry/ Immunofluorescence testing in SQSTM1 Knockout HAP1 cell line. Other related products We have a range of other formats of antibody clone [EPR18351] also available for your convenience: ab207305 , Alexa Fluor® 647 - ab225453 , PE - ab225454 , Carrier free - ab227992 Conjugation ready Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold. Compatibility This product is compatible with the Maxpar ® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar ® is a trademark of Fluidigm Canada Inc.
Host
Rabbit
Reactivity
Human
Application
WB, IP, IHC-P, Flow Cyt (Intra), ICC/IF
Platform ID
BAB042545069
Abcam
Contact
Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215
Email:
Specifications
Scientific Background
Target data Molecular adapter required for selective macroautophagy (aggrephagy) by acting as a bridge between polyubiquitinated proteins and autophagosomes (PubMed : 15340068, PubMed : 15953362, PubMed : 16286508, PubMed : 17580304, PubMed : 20168092, PubMed : 22017874, PubMed : 22622177, PubMed : 24128730, PubMed : 28404643, PubMed : 29343546, PubMed : 29507397, PubMed : 31857589, PubMed : 33509017, PubMed : 34471133, PubMed : 34893540, PubMed : 35831301, PubMed : 37306101, PubMed : 37802024). Promotes the recruitment of ubiquitinated cargo proteins to autophagosomes via multiple domains that bridge proteins and organelles in different steps (PubMed : 16286508, PubMed : 20168092, PubMed : 22622177, PubMed : 24128730, PubMed : 28404643, PubMed : 29343546, PubMed : 29507397, PubMed : 34893540, PubMed : 37802024). SQSTM1 first mediates the assembly and removal of ubiquitinated proteins by undergoing liquid-liquid phase separation upon binding to ubiquitinated proteins via its UBA domain, leading to the formation of insoluble cytoplasmic inclusions, known as p62 bodies (PubMed : 15911346, PubMed : 20168092, PubMed : 22017874, PubMed : 24128730, PubMed : 29343546, PubMed : 29507397, PubMed : 31857589, PubMed : 37802024). SQSTM1 then interacts with ATG8 family proteins on autophagosomes via its LIR motif, leading to p62 body recruitment to autophagosomes, followed by autophagic clearance of ubiquitinated proteins (PubMed : 16286508, PubMed : 17580304, PubMed : 20168092, PubMed : 22622177, PubMed : 24128730, PubMed : 28404643, PubMed : 37802024). SQSTM1 is itself degraded along with its ubiquitinated cargos (PubMed : 16286508, PubMed : 17580304, PubMed : 37802024). Also required to recruit ubiquitinated proteins to PML bodies in the nucleus (PubMed : 20168092). Also involved in autophagy of peroxisomes (pexophagy) in response to reactive oxygen species (ROS) by acting as a bridge between ubiquitinated PEX5 receptor and autophagosomes (PubMed : 26344566). Acts as an activator of the NFE2L2/NRF2 pathway via interaction with KEAP1 : interaction inactivates the BCR(KEAP1) complex by sequestering the complex in inclusion bodies, promoting nuclear accumulation of NFE2L2/NRF2 and subsequent expression of cytoprotective genes (PubMed : 20452972, PubMed : 28380357, PubMed : 33393215, PubMed : 37306101). Promotes relocalization of 'Lys-63'-linked ubiquitinated STING1 to autophagosomes (PubMed : 29496741). Involved in endosome organization by retaining vesicles in the perinuclear cloud : following ubiquitination by RNF26, attracts specific vesicle-associated adapters, forming a molecular bridge that restrains cognate vesicles in the perinuclear region and organizes the endosomal pathway for efficient cargo transport (PubMed : 27368102, PubMed : 33472082). Sequesters tensin TNS2 into cytoplasmic puncta, promoting TNS2 ubiquitination and proteasomal degradation (PubMed : 25101860). May regulate the activation of NFKB1 by TNF, nerve growth factor (NGF) and interleukin-1 (PubMed : 10356400, PubMed : 10747026, PubMed : 11244088, PubMed : 12471037, PubMed : 16079148, PubMed : 19931284). May play a role in titin/TTN downstream signaling in muscle cells (PubMed : 15802564). Adapter that mediates the interaction between TRAF6 and CYLD (By similarity). See full target information SQSTM1
Category Paths
- Products>Primary Antibodies>IHC Antibodies
- Products>Primary Antibodies>Recombinant Antibodies
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