Anti-Semaphorin 4D/CD100 antibody [EPR23866-124] - BSA and Azide free (Capture),Abcam,AB285026

ab285026 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human Semaphorin 4D/CD100. The recommended pair for sandwich ELISA is: Capture: ab285026, Human Semaphorin 4D/CD100 Capture Antibody (unconjugated) Detector: ab285132 , Human Semaphorin 4D/CD100 Detector Antibody (unconjugated) The reference range value is 78 - 5000 pg/mL. Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies . Conjugation ready Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold. Sandwich ELISA The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Host

Rabbit

Reactivity

Human

Application

sELISA

Platform ID

BAB780126033

Abcam

Headquarters

Discovery Drive Cambridge Biomedical Campus Cambridge CB2 0AX UK

Contact

Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215

Product Specifications
Scientific Background

Specifications

NameAnti-Semaphorin 4D/CD100 antibody [EPR23866-124] - BSA and Azide free (Capture)
Cat. No.AB285026
HostRabbit
IsotypeIgG
ReactivityHuman
ApplicationsELISA
ClonalityMonoclonal
Clone NumberEPR23866-124
Concentration0.998 - 1.018 mg/mL Batch dependent concentration
ImmunogenThe exact immunogen used to generate this antibody is proprietary information.
PurityAffinity purification Protein A
Appearance/FormLiquid
ShippingBlue Ice
FormulationConstituents: PBS
Storage+4°C
Regulatory StatusResearch Use Only

Scientific Background

Target data Cell surface receptor for PLXNB1 and PLXNB2 that plays an important role in cell-cell signaling (PubMed : 20877282). Regulates GABAergic synapse development (By similarity). Promotes the development of inhibitory synapses in a PLXNB1-dependent manner (By similarity). Modulates the complexity and arborization of developing neurites in hippocampal neurons by activating PLXNB1 and interaction with PLXNB1 mediates activation of RHOA (PubMed : 19788569). Promotes the migration of cerebellar granule cells (PubMed : 16055703). Plays a role in the immune system; induces B-cells to aggregate and improves their viability (in vitro) (PubMed : 8876214). Induces endothelial cell migration through the activation of PTK2B/PYK2, SRC, and the phosphatidylinositol 3-kinase-AKT pathway (PubMed : 16055703). See full target information SEMA4D

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