Anti-Smad2 antibody [EP784Y],Abcam,AB40855

What is this antibody validated in? Anti-Smad2 antibody [EP784Y] (ab40855) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Rat samples. What is the molecular weight of Smad2? Anti-Smad2 [EP784Y] (ab40855) specifically detects a band for Smad2 (UniProt: Q15796) at a molecular weight of 58kDa. Trusted by the scientific community Anti-Smad2 [EP784Y] (ab40855) was first used in a scientific publication in 2006 and has been cited over 100 times in peer-reviewed journals. Trial sizes available! Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies. Specificity confirmed The specificity of Anti-Smad2 antibody [EP784Y] (ab40855) has been confirmed by Western blot testing in SMAD2 Knockout HeLa cells. Other related products We have a range of other formats of antibody clone [EP784Y] also available for your convenience: ab40855, Carrier free - ab157371 , PE - ab212096 , Alexa Fluor® 488 - ab215913 Species reactivity Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information. Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies .

Host

Rabbit

Reactivity

Rat, Human

Application

WB, ICC/IF, IHC-P, Flow Cyt (Intra), IP, ChIC/CUT&RUN-seq

Platform ID

BAB599578944

Abcam

Headquarters

Discovery Drive Cambridge Biomedical Campus Cambridge CB2 0AX UK

Contact

Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215

Product Specifications
Scientific Background

Specifications

NameAnti-Smad2 antibody [EP784Y]
Cat. No.AB40855
HostRabbit
IsotypeIgG
ReactivityRat, Human
ApplicationWB, ICC/IF, IHC-P, Flow Cyt (Intra), IP, ChIC/CUT&RUN-seq
ClonalityMonoclonal
Clone NumberEP784Y
Concentration0.51 mg/mL Batch dependent concentration
ImmunogenThe exact immunogen used to generate this antibody is proprietary information.
PurityAffinity purification Protein A
Appearance/FormLiquid
ShippingBlue Ice
FormulationpH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Storage-20°C
Regulatory StatusResearch Use Only

Scientific Background

Target data Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. Promotes TGFB1-mediated transcription of odontoblastic differentiation genes in dental papilla cells (By similarity). Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. May act as a tumor suppressor in colorectal carcinoma (PubMed : 8752209). See full target information SMAD2

Category Paths

Request a product

Please provide the required information below so that we can quickly source your products.