Anti-Smad2 (phospho T8) + Smad3 (phospho T8) antibody [EPR23682-64],Abcam,AB254407

What is this antibody validated in? Anti-Smad2 (phospho T8) + Smad3 (phospho T8) antibody [EPR23682-64] (ab254407) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry/immunofluorescence (ICC/IF), Dot Blot in Human, Mouse, Rat samples. Trusted by the scientific community Anti-Smad2 (phospho T8) + Smad3 (phospho T8) [EPR23682-64] (ab254407) was first used in a scientific publication in 2020 and has been cited over 10 times in peer-reviewed journals. Trial sizes available! Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies. Other related products We have a range of other formats of antibody clone [EPR23682-64] also available for your convenience: ab254407, Carrier free - ab276140 , PE - ab318411 , APC - ab318514 , Alexa Fluor® 488 - ab318617 , Alexa Fluor® 647 - ab318720 , Alexa Fluor® 594 - ab318823 , Alexa Fluor® 555 - ab318923 Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies .

Host

Rabbit

Reactivity

Mouse, Rat, Human

Application

ICC/IF, IP, ChIC/CUT&RUN-seq, WB, Dot

Platform ID

BAB537197637

Abcam

Headquarters

Discovery Drive Cambridge Biomedical Campus Cambridge CB2 0AX UK

Contact

Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215

Product Specifications
Scientific Background

Specifications

NameAnti-Smad2 (phospho T8) + Smad3 (phospho T8) antibody [EPR23682-64]
Cat. No.AB254407
HostRabbit
IsotypeIgG
ReactivityMouse, Rat, Human
ApplicationICC/IF, IP, ChIC/CUT&RUN-seq, WB, Dot
ClonalityMonoclonal
Clone NumberEPR23682-64
Concentration0.487 mg/mL Batch dependent concentration
ImmunogenThe exact immunogen used to generate this antibody is proprietary information.
PurityAffinity purification Protein A
Appearance/FormLiquid
ShippingBlue Ice
FormulationPreservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Storage-20°C
Regulatory StatusResearch Use Only

Scientific Background

Target data Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. Promotes TGFB1-mediated transcription of odontoblastic differentiation genes in dental papilla cells (By similarity). Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. May act as a tumor suppressor in colorectal carcinoma (PubMed : 8752209). See full target information SMAD2 phospho T8

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