Anti-Smad3 antibody [EP568Y],Abcam,AB40854

What is this antibody validated in? Anti-Smad3 antibody [EP568Y] (ab40854) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunocytochemistry/immunofluorescence (ICC/IF), ELISA in Human, Rat samples. What is the molecular weight of Smad3? Anti-Smad3 [EP568Y] (ab40854) specifically detects a band for Smad3 (UniProt: P84022) at a molecular weight of 48kDa. Trusted by the scientific community Anti-Smad3 [EP568Y] (ab40854) was first used in a scientific publication in 2006 and has been cited over 300 times in peer-reviewed journals. Reviewed by scientists Anti-Smad3 [EP568Y] (ab40854) has over 10 independent reviews from customers. Trial sizes available! Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies. Specificity confirmed The specificity of Anti-Smad3 antibody [EP568Y] (ab40854) has been confirmed by Western blot testing in SMAD3 Knockout A549 cells. Other related products We have a range of other formats of antibody clone [EP568Y] also available for your convenience: ab40854, Carrier free - ab157372 , Alexa Fluor® 488 - ab204257 , Alexa Fluor® 647 - ab204461 , HRP - ab204462 , Alexa Fluor® 594 - ab206350 , Alexa Fluor® 555 - ab206582 , PE - ab208751 Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies . Shipping conditions update: ambient shipping This product will be delivered at ambient temperature instead of chilled – this is by design. Extensive stability testing confirmed that our products are suitable for shipment under ambient conditions and maintain expected quality. Why the change? It’s part of our commitment to more sustainable packaging solutions, with ambient deliveries using eco-friendly materials such as recyclable cardboard instead of polystyrene. What you need to know Ambient shipments come clearly marked on the delivery note. No ice will be included in ambient shipments, but mixed orders (ambient and cold-chain items) will still arrive with ice packs to protect temperature-sensitive products. Warranty coverage remains fully valid, aligned with our validated shipping method. Please store the product as per the datasheet instructions upon receipt. Find out more - https://www.abcam.com/en-us/support/shipping-storage-support/ambient-shipping

Host

Rabbit

Reactivity

Rat, Human

Application

ChIC/CUT&RUN-seq, sELISA, WB, Flow Cyt (Intra), ICC/IF

Platform ID

BAB912285095

Abcam

Headquarters

Discovery Drive Cambridge Biomedical Campus Cambridge CB2 0AX UK

Contact

Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215

Product Specifications
Scientific Background

Specifications

NameAnti-Smad3 antibody [EP568Y]
Cat. No.AB40854
HostRabbit
IsotypeIgG
ReactivityRat, Human
ApplicationChIC/CUT&RUN-seq, sELISA, WB, Flow Cyt (Intra), ICC/IF
ClonalityMonoclonal
Clone NumberEP568Y
Concentration1.136 mg/mL Batch dependent concentration
ImmunogenThe exact immunogen used to generate this antibody is proprietary information.
PurityAffinity purification Protein A
Appearance/FormLiquid
ShippingConditional Ambient
FormulationpH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Storage-20°C
Regulatory StatusResearch Use Only

Scientific Background

Target data Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. See full target information SMAD3

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