Anti-TNF alpha antibody [EPR19147],Abcam,AB183218
Product Specifications Anti-TNF alpha antibody [EPR19147] (ab183218) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in ELISA, ICC/IF, IP, WB in human, mouse samples. Anti-TNF alpha antibody [EPR19147] (ab183218) specifically detects TNF alpha (UniProt ID: P01375; Molecular weight: 26kDa) and is sold in 100 µL and 1 mL selling sizes. Quality and Validation Abcam's high quality manufacturing and validation processes ensure Anti-TNF alpha antibody [EPR19147] (ab183218) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility. The specificity of Anti-TNF alpha antibody [EPR19147] (ab183218) has been confirmed by testing in knockout samples. Anti-TNF alpha antibody [EPR19147] (ab183218) has been cited over 104 times in peer reviewed journals and is trusted by the scientific community. Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies .
Host
Rabbit
Reactivity
Mouse, Human
Application
IP, WB, ELISA, ICC/IF
Platform ID
BAB747030662
Abcam
Contact
Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215
Email:
Specifications
Scientific Background
Target data The TNF protein, primarily secreted by macrophages, binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It induces cell death in specific tumor cell lines and acts as a potent pyrogen, causing fever directly or by stimulating interleukin-1 secretion. TNF is implicated in cachexia induction and can stimulate cell proliferation and differentiation under certain conditions. It impairs regulatory T-cells (Treg) function in rheumatoid arthritis patients through FOXP3 dephosphorylation, upregulating protein phosphatase 1 (PP1), which dephosphorylates 'Ser-418' of FOXP3, inactivating FOXP3 and leading to defective Treg cells. TNF is a key mediator of cell death in the anticancer effect of BCG-stimulated neutrophils with DIABLO/SMAC mimetic in the RT4v6 bladder cancer cell line. It induces insulin resistance in adipocytes by inhibiting insulin-induced IRS1 tyrosine phosphorylation and glucose uptake. TNF plays a role in angiogenesis by inducing VEGF production with IL1B and IL6. Additionally, the TNF intracellular domain (ICD) form stimulates IL12 production in dendritic cells. This supplementary information is collated from multiple sources and compiled automatically. See full target information TNF
Category Paths
- Products>Primary Antibodies>Monoclonal Antibodies
- Products>Primary Antibodies>Recombinant Antibodies
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