Anti-TrkB antibody [EPR18925-48] - BSA and Azide free (Detector),Abcam,AB242343
ab242343 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human TrkB. The recommended pair for sandwich ELISA is: Capture: ab267457 , Human TrkB Capture Antibody (unconjugated) Detector: ab242343, Human TrkB Detector Antibody (unconjugated) The reference range value is 54.6875 - 3500 pg/ml. Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies . Conjugation ready Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold. Sandwich ELISA The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Host
Rabbit
Reactivity
Human
Application
sELISA
Platform ID
BAB689447051
Abcam
Contact
Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215
Email:
Specifications
Scientific Background
Target data The protein expressed by the NTRK2 gene functions as a receptor for BDNF/brain-derived neurotrophic factor and NTF4/neurotrophin-4, and can also bind to NTF3/neurotrophin-3, although this interaction is less efficient. Upon binding with its ligands, the receptor undergoes homodimerization, autophosphorylation, and activation, enabling it to recruit, phosphorylate, and/or activate several downstream effectors like SHC1, FRS2, SH2B1, SH2B2, and PLCG1. These effectors regulate distinct but overlapping signaling cascades. Through SHC1, FRS2, SH2B1, and SH2B2, NTRK2 activates the GRB2-Ras-MAPK cascade, which regulates neuronal differentiation including neurite outgrowth, as well as the Ras-PI3 kinase-AKT1 signaling cascade, which is primarily involved in growth and survival. The PLCG1 pathway, which influences synaptic plasticity, plays a role in learning and memory by affecting both short-term synaptic function and long-term potentiation. Additionally, PLCG1 activation leads to NF-Kappa-B activation, promoting the transcription of genes related to cell survival, thereby suppressing anoikis. NTRK2 may also be involved in neurotrophin-dependent calcium signaling in glial cells and in mediating communication between neurons and glial cells. This supplementary information is collated from multiple sources and compiled automatically. See full target information NTRK2
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