Anti-VCAM1 antibody [EPR5047],Abcam,AB134047

Anti-VCAM1 antibody [EPR5047] (ab134047) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt (Intra), I-ELISA, ICC/IF, IHC-P, IP and WB. Anti-VCAM1 antibody [EPR5047] (ab134047) was first used in a scientific publication in 2013 and has been cited over 309 times in peer reviewed journals. It's performance in Western Blot and IHC in mouse and human samples is trusted by the scientific community. Abcam's high quality manufacturing and validation processes ensure Anti-VCAM1 antibody [EPR5047] (ab134047) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility. The specificity of Anti-VCAM1 antibody [EPR5047] (ab134047) has been confirmed by Western Blot testing in VCAM1 knockout A549 cells ( ab273758 ). Anti-VCAM1 antibody [EPR5047] (ab134047) has 16 independent reviews from customers. Anti-VCAM1 antibody [EPR5047] (ab134047) specifically detects VCAM1 (UniProt ID: P29533; Molecular weight: 79kDa) and is sold in 100 µL and 1 mL selling sizes. Conjugation-ready, carrier free format available for antibody clone EPR5047 - ab215380 . Antibody clone EPR5047 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor ® 647, HRP, PE, FITC, APC ( ab194319 , ab195540 , ab223981 , ab223982 , ab223983 ). - Specificity confirmed with SYP knockout cell line validation Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies .

Host

Rabbit

Reactivity

Mouse, Rat, Human

Application

ICC/IF, IP, WB, IHC-P, I-ELISA, Flow Cyt (Intra)

Platform ID

BAB635061904

Abcam

Headquarters

Discovery Drive Cambridge Biomedical Campus Cambridge CB2 0AX UK

Contact

Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215

Product Specifications
Scientific Background

Specifications

NameAnti-VCAM1 antibody [EPR5047]
Cat. No.AB134047
HostRabbit
IsotypeIgG
ReactivityMouse, Rat, Human
ApplicationICC/IF, IP, WB, IHC-P, I-ELISA, Flow Cyt (Intra)
ClonalityMonoclonal
Clone NumberEPR5047
Concentration0.422 mg/mL Batch dependent concentration
ImmunogenThe exact immunogen used to generate this antibody is proprietary information.
PurityAffinity purification Protein A
Appearance/FormLiquid
ShippingBlue Ice
FormulationpH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Storage-20°C
Regulatory StatusResearch Use Only

Scientific Background

Target data Cell adhesion glycoprotein predominantly expressed on the surface of endothelial cells that plays an important role in immune surveillance and inflammation (PubMed : 31310649). Acts as a major regulator of leukocyte adhesion to the endothelium through interaction with different types of integrins (PubMed : 10209034). During inflammatory responses, binds ligands on the surface of activated endothelial cells to initiate the activation of calcium channels and the plasma membrane-associated small GTPase RAC1 leading to leukocyte transendothelial migration (PubMed : 22970700). Also serves as a quality-control checkpoint for entry into bone marrow by providing a 'don't-eat-me' stamping in the context of major histocompatibility complex (MHC) class-I presentation (PubMed : 35210567). See full target information VCAM1

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