Anti-VDAC1/Porin antibody [EPR27552-6] - Mitochondrial Loading Control,Abcam,AB306581
What is this antibody validated in? Anti-VDAC1/Porin antibody [EPR27552-6] (ab306581) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Dot Blot in Human, Mouse, Rat samples. What is the molecular weight of VDAC1/Porin? Anti-VDAC1/Porin [EPR27552-6] (ab306581) specifically detects a band for VDAC1/Porin (UniProt: P21796) at a molecular weight of 31kDa. Trial sizes available! Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies. Specificity confirmed The specificity of Anti-VDAC1/Porin antibody [EPR27552-6] (ab306581) has been confirmed by Western blot testing in VDAC1 Knockout HAP1 cells. Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies .
Host
Rabbit
Reactivity
Mouse, Human, Rat
Application
WB, IHC-P, Dot
Platform ID
BAB322655609
Abcam
Contact
Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215
Email:
Specifications
Scientific Background
Target data Non-selective voltage-gated ion channel that mediates the transport of anions and cations through the mitochondrion outer membrane and plasma membrane (PubMed : 10661876, PubMed : 11845315, PubMed : 18755977, PubMed : 30061676, PubMed : 8420959). The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis (PubMed : 10661876, PubMed : 11845315, PubMed : 18755977, PubMed : 8420959). It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV (PubMed : 10661876, PubMed : 18755977, PubMed : 8420959). The open state has a weak anion selectivity whereas the closed state is cation-selective (PubMed : 18755977, PubMed : 8420959). Binds various signaling molecules, including the sphingolipid ceramide, the phospholipid phosphatidylcholine, and the sterols cholesterol and oxysterol (PubMed : 18755977, PubMed : 31015432). In depolarized mitochondria, acts downstream of PRKN and PINK1 to promote mitophagy or prevent apoptosis; polyubiquitination by PRKN promotes mitophagy, while monoubiquitination by PRKN decreases mitochondrial calcium influx which ultimately inhibits apoptosis (PubMed : 32047033). May participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis (PubMed : 15033708, PubMed : 25296756). May mediate ATP export from cells (PubMed : 30061676). Part of a complex composed of HSPA9, ITPR1 and VDAC1 that regulates mitochondrial calcium-dependent apoptosis by facilitating calcium transport from the ER lumen to the mitochondria intermembrane space thus providing calcium for the downstream calcium channel MCU that directly releases it into mitochondria matrix (By similarity). Mediates cytochrome c efflux (PubMed : 20230784).. Catalyzes the scrambling of phospholipids across the outer mitochondrial membrane; the mechanism is unrelated to channel activity and is capable of translocating both anionic and zwitterionic phospholipids. See full target information VDAC1
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