Anti-Vimentin antibody [EPR3776] - BSA and Azide free,Abcam,AB193555
What is this antibody validated in? Anti-Vimentin antibody [EPR3776] - BSA and Azide free (ab193555) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF), Multiplex IHC (mIHC) in African green monkey, Human, Mouse, Rat samples. What is the molecular weight of Vimentin? Anti-Vimentin [EPR3776] - BSA and Azide free (ab193555) specifically detects a band for Vimentin (UniProt: P08670) at a molecular weight of 54kDa. Trusted by the scientific community Anti-Vimentin [EPR3776] - BSA and Azide free (ab193555) was first used in a scientific publication in 2014 and has been cited over 40 times in peer-reviewed journals. Specificity confirmed The specificity of Anti-Vimentin antibody [EPR3776] - BSA and Azide free (ab193555) has been confirmed by Immunocytochemistry/ Immunofluorescence testing in VIM Knockout HeLa cells. Other related products We have a range of other formats of antibody clone [EPR3776] also available for your convenience: ab92547 , Alexa Fluor® 594 - ab154207 , Alexa Fluor® 488 - ab185030 , Carrier free - ab193555, HRP - ab194718 , Alexa Fluor® 647 - ab194719 , Alexa Fluor® 568 - ab202504 , Alexa Fluor® 555 - ab203428 , PE - ab209446 , Alexa Fluor® 405 - ab210152 , Alexa Fluor® 568 - ab236866, Carrier free - ab307988, APC - ab314250 , ab316214 , Carrier free - ab316215 , Alexa Fluor® 750 - ab321763 , ab323238 , Carrier free - ab323247 Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies . Conjugation ready Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold. Compatibility This product is compatible with the Maxpar ® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar ® is a trademark of Fluidigm Canada Inc.
Host
Rabbit
Reactivity
Mouse, Rat, Human, African green monkey
Application
Flow Cyt (Intra), mIHC, ICC/IF, IHC-P, WB
Platform ID
BAB636561184

Abcam
Contact
Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215
Email:
Specifications
Scientific Background
Target data Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Plays a role in cell directional movement, orientation, cell sheet organization and Golgi complex polarization at the cell migration front (By similarity). Protects SCRIB from proteasomal degradation and facilitates its localization to intermediate filaments in a cell contact-mediated manner (By similarity). May promote axon outgrowth and motor fiber repair via DSP-mediated recruitment to outgrowth tips (By similarity).. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. See full target information VIM
Category Paths
- Products>Primary Antibodies>IHC Antibodies
- Products>Primary Antibodies>Recombinant Antibodies
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