Anti-Vimentin antibody [RV202] - Cytoskeleton Marker,Abcam,AB8978

Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (ab8978) is a mouse monoclonal antibody and is validated for use in Flow Cyt (Intra), ICC/IF, IHC-Fr, IHC-P, WB in mouse samples. Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (ab8978) has been cited over 570 times in peer reviewed journals and is trusted by the scientific community. Abcam's high quality validation processes ensure Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (ab8978) has high sensitivity and specificity. The specificity of Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (ab8978) has been confirmed by testing in knockout samples. Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (ab8978) has 44 independent reviews from customers. Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (ab8978) specifically detects Vimentin (UniProt ID: P08670; Molecular weight: 54kDa) and is sold in 100 µg selling sizes. Antibody clone RV202 is also available pre-conjugated to FITC for your convenience ( ab128507 ). Vimentin (57 kDa) is the intermediate filament protein (IFP) of mesenchymal cells. This IFP however often deviates from the tissue-specific and developmentally regulated pattern of expression. Besides its typical expression in most cultured cells, vimentin is also expressed together with several other IFPs during early stages of development. As differentiation proceeds, vimentin is exchanged for the tissue-specific intermediate filament type. Also in cancers, vimentin is often expressed in addition to the tissue-specific IFP. RV202 is a mouse monoclonal IgG1 antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with a cytoskeletal vimentin extract of calf lens.

Host

Mouse

Reactivity

Mouse, Rat, Human, Pig, Zebrafish

Application

WB, IHC-Fr, Flow Cyt (Intra), ICC/IF, IHC-P

Platform ID

BAB824104913

Abcam

Headquarters

Discovery Drive Cambridge Biomedical Campus Cambridge CB2 0AX UK

Contact

Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215

Product Specifications
Scientific Background

Specifications

NameAnti-Vimentin antibody [RV202] - Cytoskeleton Marker
Cat. No.AB8978
HostMouse
IsotypeIgG1
ReactivityMouse, Rat, Human, Pig, Zebrafish
ApplicationWB, IHC-Fr, Flow Cyt (Intra), ICC/IF, IHC-P
ClonalityMonoclonal
Clone NumberRV202
Concentration1 mg/mL Batch dependent concentration
ImmunogenNative Full Length Protein corresponding to Cow VIM.
PurityAffinity purification Protein G
Appearance/FormLiquid
ShippingBlue Ice
FormulationPreservative: 0.09% Sodium azide Constituents: PBS
Storage-20°C
Regulatory StatusResearch Use Only

Scientific Background

Target data Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Plays a role in cell directional movement, orientation, cell sheet organization and Golgi complex polarization at the cell migration front (By similarity). Protects SCRIB from proteasomal degradation and facilitates its localization to intermediate filaments in a cell contact-mediated manner (By similarity). May promote axon outgrowth and motor fiber repair via DSP-mediated recruitment to outgrowth tips (By similarity).. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. See full target information VIM

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