Anti-Vimentin antibody [SP20],Abcam,AB16700

What is this antibody validated in? Anti-Vimentin antibody [SP20] (ab16700) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples. What is the molecular weight of Vimentin? Anti-Vimentin [SP20] (ab16700) specifically detects a band for Vimentin (UniProt: P08670) at a molecular weight of 53kDa. Collaboration Anti-Vimentin [SP20] (ab16700) is a clone from the portfolio of Spring Bioscience (Roche) SP clones which have been optimised for immunohistochemistry (IHC). Trusted by the scientific community Anti-Vimentin [SP20] (ab16700) was first used in a scientific publication in 2005 and has been cited over 100 times in peer-reviewed journals. Reviewed by scientists Anti-Vimentin [SP20] (ab16700) has over 5 independent reviews from customers. Trial sizes available! Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies. Specificity confirmed The specificity of Anti-Vimentin antibody [SP20] (ab16700) has been confirmed by Immunocytochemistry testing in VIM Knockout HAP1 cells. Other related products We have a range of other formats of antibody clone [SP20] also available for your convenience: ab16700, ab27608 What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies .

Host

Rabbit

Reactivity

Human

Application

WB, Flow Cyt, IHC-P, ICC/IF

Platform ID

BAB449117407

Abcam

Headquarters

Discovery Drive Cambridge Biomedical Campus Cambridge CB2 0AX UK

Contact

Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215

Product Specifications
Scientific Background

Specifications

NameAnti-Vimentin antibody [SP20]
Cat. No.AB16700
HostRabbit
IsotypeIgG
ReactivityHuman
ApplicationWB, Flow Cyt, IHC-P, ICC/IF
ClonalityMonoclonal
Clone NumberSP20
Concentration0.007 mg/mL Batch dependent concentration
ImmunogenThe exact immunogen used to generate this antibody is proprietary information.
PurityAffinity purification Protein A
Appearance/FormLiquid
ShippingBlue Ice
FormulationpH: 7.2 - 7.4 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA
Storage-20°C
Regulatory StatusResearch Use Only

Scientific Background

Target data Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Plays a role in cell directional movement, orientation, cell sheet organization and Golgi complex polarization at the cell migration front (By similarity). Protects SCRIB from proteasomal degradation and facilitates its localization to intermediate filaments in a cell contact-mediated manner (By similarity). May promote axon outgrowth and motor fiber repair via DSP-mediated recruitment to outgrowth tips (By similarity).. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. See full target information VIM

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