Anti-Vimentin antibody [V9] - Cytoskeleton Marker,Abcam,AB8069

What is this antibody validated in? Anti-Vimentin antibody [V9] - Cytoskeleton Marker (ab8069) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Rat samples. What is the molecular weight of Vimentin? Anti-Vimentin [V9] - Cytoskeleton Marker (ab8069) specifically detects a band for Vimentin (UniProt: P02543) at a molecular weight of 54kDa. Trusted by the scientific community Anti-Vimentin [V9] - Cytoskeleton Marker (ab8069) was first used in a scientific publication in 2002 and has been cited over 170 times in peer-reviewed journals. Reviewed by scientists Anti-Vimentin [V9] - Cytoskeleton Marker (ab8069) has over 25 independent reviews from customers. Specificity confirmed The specificity of Anti-Vimentin antibody [V9] - Cytoskeleton Marker (ab8069) has been confirmed by Immunocytochemistry testing in VIM Knockout HAP1 cells. Other related products We have a range of other formats of antibody clone [V9] also available for your convenience: ab8069, Alexa Fluor® 488 - ab195877 , Alexa Fluor® 647 - ab195878 , HRP - ab196602 , Carrier free - ab223871 , ab283641, Carrier free - ab302471, ab316575, Carrier free - ab316907 Want a custom formulation? This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact [email protected]

Host

Mouse

Reactivity

Rat, Human

Application

Flow Cyt (Intra), IHC-P, WB, ICC/IF

Platform ID

BAB699142395

Abcam

Headquarters

Discovery Drive Cambridge Biomedical Campus Cambridge CB2 0AX UK

Contact

Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215

Product Specifications
Scientific Background

Specifications

NameAnti-Vimentin antibody [V9] - Cytoskeleton Marker
Cat. No.AB8069
HostMouse
IsotypeIgG1
ReactivityRat, Human
ApplicationFlow Cyt (Intra), IHC-P, WB, ICC/IF
ClonalityMonoclonal
Clone NumberV9
Concentration1 mg/mL Batch dependent concentration
ImmunogenThe exact immunogen used to generate this antibody is proprietary information.
PurityAffinity purification
Appearance/FormLiquid
ShippingBlue Ice
FormulationpH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 6.97% L-Arginine
Storage-20°C
Regulatory StatusResearch Use Only

Scientific Background

Target data Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Plays a role in cell directional movement, orientation, cell sheet organization and Golgi complex polarization at the cell migration front (By similarity). Protects SCRIB from proteasomal degradation and facilitates its localization to intermediate filaments in a cell contact-mediated manner (By similarity). May promote axon outgrowth and motor fiber repair via DSP-mediated recruitment to outgrowth tips (By similarity).. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. See full target information VIM

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