Anti-Vinculin antibody [EPR8185] - Loading Control,Abcam,AB129002

Anti-Vinculin antibody [EPR8185] (ab129002) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt (Intra), ICC/IF, IP and WB. Anti-Vinculin antibody [EPR8185] (ab129002) was first used in a scientific publication in 2013 and has been cited over 356 times in peer reviewed journals. It's performance in Western Blot in mouse and human samples is trusted by the scientific community. Abcam's high quality manufacturing and validation processes ensure Anti-Vinculin antibody [EPR8185] (ab129002) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility. The specificity of Anti-Vinculin antibody [EPR8185] (ab129002) has been confirmed by Western Blot testing in Vinculin knockout A431 cells. Anti-Vinculin antibody [EPR8185] (ab129002) has 21 independent reviews from customers. Vinculin antibodies are often used as loading controls in Western Blot. Anti-Vinculin antibody [EPR8185] has been verified in Western Blot samples and detects a band at 124kDa Molecular weight. Anti-Vinculin antibody [EPR8185] (ab129002) specifically detects Vinculin (UniProt ID: P18206; Molecular weight: 124kDa) and is sold in a convenient trial size to enable initial testing (20 µL) and larger sizes for subsequent scaling up experiments (100 µL, 250 µL and 1 mL). Conjugation-ready, carrier free format available for antibody clone EPR8185 - ab217171 . Antibody clone EPR8185 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor ® 488, Alexa Fluor ® 647, Alexa Fluor ® 555 ( ab196454 , ab196579 , ab206908 ). Vinculin is a focal adhesion protein that plays a critical role in cell adhesion, migration and mechanotransduction. In oncology, it influences tumor progression and metastasis by modulating the interactions between cancer cells and the extracellular matrix. Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies .

Host

Rabbit

Reactivity

Mouse, Rat, Human

Application

WB, ICC/IF, Flow Cyt (Intra), IP

Platform ID

BAB011748594

Abcam

Headquarters

Discovery Drive Cambridge Biomedical Campus Cambridge CB2 0AX UK

Contact

Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215

Product Specifications
Scientific Background

Specifications

NameAnti-Vinculin antibody [EPR8185] - Loading Control
Cat. No.AB129002
HostRabbit
IsotypeIgG
ReactivityMouse, Rat, Human
ApplicationWB, ICC/IF, Flow Cyt (Intra), IP
ClonalityMonoclonal
Clone NumberEPR8185
Concentration0.058 mg/mL Batch dependent concentration
ImmunogenThe exact immunogen used to generate this antibody is proprietary information.
PurityAffinity purification Protein A
Appearance/FormLiquid
ShippingBlue Ice
FormulationpH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Storage-20°C
Regulatory StatusResearch Use Only

Scientific Background

Target data Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion. See full target information VCL

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