Anti-WDR4 antibody,Abcam,AB241297

Target data Non-catalytic component of the METTL1-WDR4 methyltransferase complex required for the formation of N(7)-methylguanine in a subset of RNA species, such as tRNAs, mRNAs and microRNAs (miRNAs) (PubMed : 12403464, PubMed : 31031083, PubMed : 31031084, PubMed : 36599982, PubMed : 36599985, PubMed : 37369656). In the METTL1-WDR4 methyltransferase complex, WDR4 acts as a scaffold for tRNA-binding (PubMed : 36599982, PubMed : 36599985, PubMed : 37369656). Required for the formation of N(7)-methylguanine at position 46 (m7G46) in a large subset of tRNAs that contain the 5'-RAGGU-3' motif within the variable loop (PubMed : 12403464, PubMed : 34352206, PubMed : 34352207, PubMed : 36599982, PubMed : 36599985, PubMed : 37369656). M7G46 interacts with C13-G22 in the D-loop to stabilize tRNA tertiary structure and protect tRNAs from decay (PubMed : 36599982, PubMed : 36599985). Also required for the formation of N(7)-methylguanine at internal sites in a subset of mRNAs (PubMed : 31031084, PubMed : 37379838). Also required for methylation of a specific subset of miRNAs, such as let-7 (PubMed : 31031083). Independently of METTL1, also plays a role in genome stability : localizes at the DNA replication site and regulates endonucleolytic activities of FEN1 (PubMed : 26751069). See full target information WDR4