Anti-WIPI2 antibody - C-terminal,Abcam,AB229225

Target data Component of the autophagy machinery that controls the major intracellular degradation process by which cytoplasmic materials are packaged into autophagosomes and delivered to lysosomes for degradation (PubMed : 20505359, PubMed : 28561066). Involved in an early step of the formation of preautophagosomal structures (PubMed : 20505359, PubMed : 28561066). Binds and is activated by phosphatidylinositol 3-phosphate (PtdIns3P) forming on membranes of the endoplasmic reticulum upon activation of the upstream ULK1 and PI3 kinases (PubMed : 28561066). Mediates ER-isolation membranes contacts by interacting with the ULK1 : RB1CC1 complex and PtdIns3P (PubMed : 28890335). Once activated, WIPI2 recruits at phagophore assembly sites the ATG12-ATG5-ATG16L1 complex that directly controls the elongation of the nascent autophagosomal membrane (PubMed : 20505359, PubMed : 28561066).. Isoform 4. Recruits the ATG12-ATG5-ATG16L1 complex to omegasomes and preautophagosomal structures, resulting in ATG8 family proteins lipidation and starvation-induced autophagy. Isoform 4 is also required for autophagic clearance of pathogenic bacteria. Isoform 4 binds the membrane surrounding Salmonella and recruits the ATG12-5-16L1 complex, initiating LC3 conjugation, autophagosomal membrane formation, and engulfment of Salmonella. See full target information WIPI2