Anti-Werner's syndrome helicase WRN antibody [EPR6392],Abcam,AB124673
What is this antibody validated in? Anti-Werner's syndrome helicase WRN antibody [EPR6392] (ab124673) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB) in Human samples. What is the molecular weight of Werner's syndrome helicase WRN? Anti-Werner's syndrome helicase WRN [EPR6392] (ab124673) specifically detects a band for Werner's syndrome helicase WRN (UniProt: Q14191) at a molecular weight of 162kDa. Trial sizes available! Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies. Specificity confirmed The specificity of Anti-Werner's syndrome helicase WRN antibody [EPR6392] (ab124673) has been confirmed by Western blot testing in WRN Knockout HAP1 cells. Other related products We have a range of other formats of antibody clone [EPR6392] also available for your convenience: ab124673, Carrier free - ab247955 Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies .
Host
Rabbit
Reactivity
Human
Application
WB
Platform ID
BAB076828605
Abcam
Contact
Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215
Email:
Specifications
Scientific Background
Target data Multifunctional enzyme that has magnesium and ATP-dependent 3'-5' DNA-helicase activity on partially duplex substrates (PubMed : 9224595, PubMed : 9288107, PubMed : 9611231). Also has 3'->5' exonuclease activity towards double-stranded (ds)DNA with a 5'-overhang (PubMed : 11863428). Has no nuclease activity towards single-stranded (ss)DNA or blunt-ended dsDNA (PubMed : 11863428). Helicase activity is most efficient with (d)ATP, but (d)CTP will substitute with reduced efficiency; strand displacement is enhanced by single-strand binding-protein (heterotrimeric replication protein A complex, RPA1, RPA2, RPA3) (PubMed : 9611231). Binds preferentially to DNA substrates containing alternate secondary structures, such as replication forks and Holliday junctions. May play an important role in the dissociation of joint DNA molecules that can arise as products of homologous recombination, at stalled replication forks or during DNA repair. Alleviates stalling of DNA polymerases at the site of DNA lesions. Plays a role in the formation of DNA replication focal centers; stably associates with foci elements generating binding sites for RP-A (By similarity). Plays a role in double-strand break repair after gamma-irradiation (PubMed : 9224595, PubMed : 9288107, PubMed : 9611231). Unwinds some G-quadruplex DNA (d(CGG)n tracts); unwinding seems to occur in both 5'-3' and 3'-5' direction and requires a short single-stranded tail (PubMed : 10212265). d(CGG)n tracts have a propensity to assemble into tetraplex structures; other G-rich substrates from a telomeric or IgG switch sequence are not unwound (PubMed : 10212265). Depletion leads to chromosomal breaks and genome instability (PubMed : 33199508). See full target information WRN
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