Anti-Wilms Tumor Protein antibody [EPR23688-81] - BSA and Azide free (Detector),Abcam,AB281033

ab281033 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Mouse Wt1. The recommended pair for sandwich ELISA is: Capture: ab281183 , Mouse Wt1 Capture Antibody (unconjugated) Detector: ab281033, Mouse Wt1 Detector Antibody (unconjugated) The reference range value is 0.16-10 ng/mL. Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies . Conjugation ready Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold. Sandwich ELISA The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Host

Rabbit

Reactivity

Mouse

Application

sELISA

Platform ID

BAB014547250

Abcam

Headquarters

Discovery Drive Cambridge Biomedical Campus Cambridge CB2 0AX UK

Contact

Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215

Product Specifications
Scientific Background

Specifications

NameAnti-Wilms Tumor Protein antibody [EPR23688-81] - BSA and Azide free (Detector)
Cat. No.AB281033
HostRabbit
IsotypeIgG
ReactivityMouse
ApplicationsELISA
ClonalityMonoclonal
Clone NumberEPR23688-81
ImmunogenThe exact immunogen used to generate this antibody is proprietary information.
PurityAffinity purification Protein A
Appearance/FormLiquid
ShippingBlue Ice
FormulationConstituents: PBS
Storage+4°C
Regulatory StatusResearch Use Only

Scientific Background

Target data Transcription factor that plays an important role in cellular development and cell survival (PubMed : 16467207, PubMed : 16920711, PubMed : 17537799). Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3' (By similarity). Regulates the expression of numerous target genes, including EPO (PubMed : 16467207). Plays an essential role for development of the urogenital system. It has a tumor suppressor as well as an oncogenic role in tumor formation. Function may be isoform-specific : isoforms lacking the KTS motif may act as transcription factors. Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing (PubMed : 17167543). Isoform 1 has lower affinity for DNA, and can bind RNA. See full target information Wt1

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