Anti-active YAP1 antibody [EPR19812],Abcam,AB205270
Anti-active YAP1 antibody [EPR19812] (ab205270) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in ICC/IF, IHC-P and WB. Abcam's high quality manufacturing and validation processes ensure Anti-active YAP1 antibody [EPR19812] (ab205270) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility. The specificity of Anti-active YAP1 antibody [EPR19812] (ab205270) has been confirmed by Western Blot testing in active YAP1 knockout HAP1 cells. Anti-active YAP1 antibody [EPR19812] (ab205270) has 25 independent reviews from customers. Anti-active YAP1 antibody [EPR19812] (ab205270) specifically detects active YAP1 (UniProt ID: P46937; Molecular weight: 55kDa) and is sold in a convenient trial size to enable initial testing (20 µL) and larger sizes for subsequent scaling up experiments (100 µL and 1 mL). Conjugation-ready, carrier free format available for antibody clone EPR19812 - ab223126 . Antibody clone EPR19812 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor ® 647, Alexa Fluor ® 488, Alexa Fluor ® 594, Alexa Fluor ® 568, Alexa Fluor ® 555, Alexa Fluor ® 750 ( ab225440 , ab225441 , ab311764 , ab313045 , ab313246 , ab321359 ). YAP1 is crucial in disease as it regulates cell proliferation, differentiation and survival, playing significant roles in cancer, liver diseases and orthopaedic degenerative conditions. Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies .
Host
Rabbit
Reactivity
Mouse, Human
Application
ICC/IF, WB, IHC-P
Platform ID
BAB207655893
Abcam
Contact
Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215
Email:
Specifications
Scientific Background
Target data The protein expressed by the YAP1 gene functions as a transcriptional regulator, acting both as a coactivator and corepressor. It is a critical downstream regulatory target in the Hippo signaling pathway, which is crucial for organ size control and tumor suppression, as it restricts proliferation and promotes apoptosis. The pathway involves a kinase cascade where STK3/MST2 and STK4/MST1 activate LATS1/2, which then inactivates the YAP1 oncoprotein. YAP1 plays a key role in tissue tension and 3D tissue shape by regulating cortical actomyosin network formation through ARHGAP18. It controls cell proliferation in response to cell contact, with its phosphorylation by LATS1/2 preventing nuclear translocation necessary for regulating cellular genes linked to proliferation, death, and migration. TEAD transcription factors are needed for YAP1 to stimulate gene expression, cell growth, anchorage-independent growth, and EMT induction. Additionally, YAP1 suppresses ciliogenesis by repressing TEAD4 target genes AURKA and PLK1. Isoforms 2 and 3 of YAP1 activate the C-terminal fragment of ERBB4 (isoform 3). This supplementary information is collated from multiple sources and compiled automatically. See full target information YAP1
Category Paths
- Products>Primary Antibodies>Monoclonal Antibodies
- Products>Primary Antibodies>IHC Antibodies
- Products>Primary Antibodies>Recombinant Antibodies
- Products>Trial Size Antibodies
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