Anti-alpha Tubulin antibody [DM1A] - Loading Control,Abcam,AB7291

Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) is a mouse monoclonal antibody and is validated for use in Flow Cyt (Intra), ICC/IF, IHC-P and WB. Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) was first used in a scientific publication in 2005 and has been cited over 1224 times in peer reviewed journals. It's performance in Western blot in human, mouse and rat samples is trusted by the scientific community. Abcam's high quality validation processes ensure Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) has high sensitivity and specificity. Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) has 91 independent reviews from customers. alpha Tubulin antibodies are often used as loading controls in Western Blot. Anti-alpha Tubulin antibody [DM1A] - Loading Control has been verified in Western Blot samples and detects a band at 51kDa Molecular weight. Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) specifically detects alpha Tubulin (UniProt ID: P02552; Molecular weight: 51kDa) and is sold in 100 µg and 250 µg selling sizes. Conjugation-ready, carrier free format available for antibody clone DM1A - ab264493 . Antibody clone DM1A is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor ® 68, Alexa Fluor ® 79, Alexa Fluor ® 488, Alexa Fluor ® 594, HRP, FITC ( ab18493 , ab184577 , ab195887 , ab195889 , ab4742 ). Alpha-Tubulin (α-Tubulin, TUBBA) is a fundamental protein that forms microtubules, essential components of the cytoskeleton in eukaryotic cells. Microtubules, composed of α- and β-tubulin heterodimers, play a crucial role in maintaining cell shape, enabling intracellular transport and facilitating cell division. Due to its ubiquitous presence and stability, α-Tubulin is commonly used as a loading control in western blotting (WB) and other protein quantification assays. Understanding α-Tubulin's functions and interactions is vital for studying cellular dynamics, mitosis and various physiological processes

Host

Mouse

Reactivity

Mouse, Rat, Human

Application

IHC-P, ICC/IF, WB, Flow Cyt (Intra)

Platform ID

BAB951122766

Abcam

Headquarters

Discovery Drive Cambridge Biomedical Campus Cambridge CB2 0AX UK

Contact

Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215

Product Specifications
Scientific Background

Specifications

NameAnti-alpha Tubulin antibody [DM1A] - Loading Control
Cat. No.AB7291
HostMouse
IsotypeIgG1
ReactivityMouse, Rat, Human
ApplicationIHC-P, ICC/IF, WB, Flow Cyt (Intra)
ClonalityMonoclonal
Clone NumberDM1A
Concentration1 mg/mL Batch dependent concentration
ImmunogenThe exact immunogen used to generate this antibody is proprietary information.
PurityAffinity purification
Appearance/FormLiquid
ShippingBlue Ice
FormulationpH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 6.97% L-Arginine
Storage-20°C
Regulatory StatusResearch Use Only

Scientific Background

Target data Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin. See full target information TUBA1A

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