Anti-alpha-hemolysin antibody [8B7] - N-terminal,Abcam,AB190467

What is this antibody validated in? Anti-alpha-hemolysin antibody [8B7] - N-terminal (ab190467) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Neutralising in Staphylococcus aureus samples. What is the molecular weight of alpha-hemolysin? Anti-alpha-hemolysin [8B7] - N-terminal (ab190467) specifically detects a band for alpha-hemolysin (UniProt: P09616) at a molecular weight of 36kDa. Trusted by the scientific community Anti-alpha-hemolysin [8B7] - N-terminal (ab190467) was first used in a scientific publication in 2014 and has been cited over 10 times in peer-reviewed journals.

Host

Mouse

Reactivity

Staphylococcus aureus

Application

FuncS (Neut/Block), WB

Platform ID

BAB200448090

Abcam

Headquarters

Discovery Drive Cambridge Biomedical Campus Cambridge CB2 0AX UK

Contact

Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215

Product Specifications
Scientific Background

Specifications

NameAnti-alpha-hemolysin antibody [8B7] - N-terminal
Cat. No.AB190467
HostMouse
IsotypeIgG3
ReactivityStaphylococcus aureus
ApplicationFuncS (Neut/Block), WB
ClonalityMonoclonal
Clone Number8B7
Concentration1.68 mg/mL Batch dependent concentration
ImmunogenSynthetic Peptide within Staphylococcus aureus hly. The exact immunogen used to generate this antibody is proprietary information.
PurityAffinity purification Protein A
Appearance/FormLiquid
ShippingBlue Ice
FormulationConstituents: PBS
Storage-20°C
Regulatory StatusResearch Use Only

Scientific Background

Target data Alpha-toxin binds to the membrane of eukaryotic cells (particularly red blood cells, RBC) forming pores, resulting in hemolysis, with the release of low-molecular weight molecules leading to eventual osmotic RBC lysis (PubMed : 1587866, PubMed : 20624979). Human RBCs bind much less alpha-toxin than do rabbit RBCs (PubMed : 1587866, PubMed : 20624979). Heptamer oligomerization and pore formation is required for lytic activity (PubMed : 1587866, PubMed : 20624979). See full target information hly Additional targets alpha-hemolysin

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