Anti-beta Actin antibody - Loading Control,Abcam,AB8227

Anti-beta Actin antibody (ab8227) is a rabbit polyclonal antibody and is validated for use in ICC/IF, IHC-P and WB. Anti-beta Actin antibody (ab8227) was first used in a scientific publication in 1991 and has been cited over 4031 times in peer reviewed journals. It's performance in Western blot in human, mouse and rat samples is trusted by the scientific community. Abcam's high quality validation processes ensure Anti-beta Actin antibody (ab8227) has high sensitivity and specificity. Anti-beta Actin antibody (ab8227) has 104 independent reviews from customers. beta Actin antibodies are often used as loading controls in Western Blot. Anti-beta Actin antibody has been verified in Western Blot samples and detects a band at 42kDa Molecular weight. Anti-beta Actin antibody (ab8227) specifically detects beta Actin (UniProt ID: P60709; Molecular weight: 42kDa) and is sold in 50 µg selling sizes. Beta-Actin (β-actin) is a highly conserved protein that plays a crucial role in cell motility, structure and integrity. It is one of the six actin isoforms found in humans and is a major component of the cytoskeleton in non-muscle cells. β-actin is involved in various cellular processes, including cell division, signaling and maintaining cell shape. Due to its ubiquitous expression and stability, β-actin is commonly used as a loading control in western blotting (WB) and other protein quantification assays. Understanding β-actin's functions and interactions is essential for studying cellular dynamics and various physiological processes.

Host

Rabbit

Reactivity

Mouse, Rat, Rabbit, Chicken, Cow, Dog, Human, Xenopus laevis, Fish, Chinese hamster

Application

WB, IHC-P, ICC/IF

Platform ID

BAB286617973

Abcam

Headquarters

Discovery Drive Cambridge Biomedical Campus Cambridge CB2 0AX UK

Contact

Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215

Product Specifications
Scientific Background

Specifications

NameAnti-beta Actin antibody - Loading Control
Cat. No.AB8227
HostRabbit
IsotypeIgG
ReactivityMouse, Rat, Rabbit, Chicken, Cow, Dog, Human, Xenopus laevis, Fish, Chinese hamster
ApplicationWB, IHC-P, ICC/IF
ClonalityPolyclonal
Concentration1 mg/mL Batch dependent concentration
ImmunogenThe exact immunogen used to generate this antibody is proprietary information.
PurityAffinity purification Immunogen
Appearance/FormLiquid
ShippingBlue Ice
FormulationpH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
Storage-20°C
Regulatory StatusResearch Use Only

Scientific Background

Target data Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells (PubMed : 25255767, PubMed : 29581253). Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction (PubMed : 29581253). In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA (PubMed : 29925947). Plays a role in the assembly of the gamma-tubulin ring complex (gTuRC), which regulates the minus-end nucleation of alpha-beta tubulin heterodimers that grow into microtubule protafilaments (PubMed : 39321809, PubMed : 38609661). Part of the ACTR1A/ACTB filament around which the dynactin complex is built (By similarity). The dynactin multiprotein complex activates the molecular motor dynein for ultra-processive transport along microtubules (By similarity). See full target information ACTB

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