Anti-beta Catenin antibody [E247] - ChIP Grade,Abcam,AB32572
Anti-beta Catenin antibody [E247] - ChIP Grade (ab32572) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in ChIP, ICC/IF, IHC-P, IP and WB. Anti-beta Catenin antibody [E247] - ChIP Grade (ab32572) was first used in a scientific publication in 1999 and has been cited over 1076 times in peer reviewed journals. It's performance in Western Blot in human, mouse and rat samples is trusted by the scientific community. Abcam's high quality manufacturing and validation processes ensure Anti-beta Catenin antibody [E247] - ChIP Grade (ab32572) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility. The specificity of Anti-beta Catenin antibody [E247] - ChIP Grade (ab32572) has been confirmed by Western Blot testing in beta Catenin knockout HepG2 cells ( ab277911 ). Anti-beta Catenin antibody [E247] - ChIP Grade (ab32572) has 75 independent reviews from customers. Anti-beta Catenin antibody [E247] - ChIP Grade (ab32572) specifically detects beta Catenin (UniProt ID: P35222; Molecular weight: 86kDa) and is sold in a convenient trial size to enable initial testing (20 µL) and larger sizes for subsequent scaling up experiments (100 µL and 1 mL). Conjugation-ready, carrier free format available for antibody clone E247 - ab196204 . Antibody clone E247 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor ® 488, Alexa Fluor ® 647, HRP, Alexa Fluor ® 594, Alexa Fluor ® 555 ( ab194118 , ab194119 , ab194120 , ab201771 , ab202496 ). Top cited antibody on the market with >1370 citations. E247 is top rated with >75 reviews. Beta-catenin plays a crucial role in cell adhesion and gene transcription and its dysregulation is linked to various cancers and diseases including, myocardial infarction, arrhythmias, cardiomyopathie and Alzheimers disease Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies .
Host
Rabbit
Reactivity
Mouse, Rat, Human
Application
IHC-P, mIHC, WB, ChIP, ChIC/CUT&RUN-seq, IP, ICC/IF
Platform ID
BAB912130227
Abcam
Contact
Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215
Email:
Specifications
Scientific Background
Target data Key downstream component of the canonical Wnt signaling pathway (PubMed : 17524503, PubMed : 18077326, PubMed : 18086858, PubMed : 18957423, PubMed : 21262353, PubMed : 22155184, PubMed : 22647378, PubMed : 22699938). In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome (PubMed : 17524503, PubMed : 18077326, PubMed : 18086858, PubMed : 18957423, PubMed : 21262353, PubMed : 22155184, PubMed : 22647378, PubMed : 22699938). In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes (PubMed : 17524503, PubMed : 18077326, PubMed : 18086858, PubMed : 18957423, PubMed : 21262353, PubMed : 22155184, PubMed : 22647378, PubMed : 22699938). Involved in the regulation of cell adhesion, as component of an E-cadherin : catenin adhesion complex (By similarity). Acts as a negative regulator of centrosome cohesion (PubMed : 18086858). Involved in the CDK2/PTPN6/CTNNB1/CEACAM1 pathway of insulin internalization (PubMed : 21262353). Blocks anoikis of malignant kidney and intestinal epithelial cells and promotes their anchorage-independent growth by down-regulating DAPK2 (PubMed : 18957423). Disrupts PML function and PML-NB formation by inhibiting RANBP2-mediated sumoylation of PML (PubMed : 22155184). Promotes neurogenesis by maintaining sympathetic neuroblasts within the cell cycle (By similarity). Involved in chondrocyte differentiation via interaction with SOX9 : SOX9-binding competes with the binding sites of TCF/LEF within CTNNB1, thereby inhibiting the Wnt signaling (By similarity). Acts as a positive regulator of odontoblast differentiation during mesenchymal tooth germ formation, via promoting the transcription of differentiation factors such as LEF1, BMP2 and BMP4 (By similarity). Activity is repressed in a MSX1-mediated manner at the bell stage of mesenchymal tooth germ formation which prevents premature differentiation of odontoblasts (By similarity). See full target information CTNNB1
Category Paths
- Products>Primary Antibodies>Monoclonal Antibodies
- Products>Primary Antibodies>IHC Antibodies
- Products>Primary Antibodies>Recombinant Antibodies
- Products>Trial Size Antibodies
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