Anti-c-Myc antibody [Y69] - ChIP Grade,Abcam,AB32072

Anti-c-Myc antibody [Y69] - ChIP Grade (ab32072) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in ChIC/CUT&RUN-seq, ChIP,-seq Flow Cyt (Intra), ICC/IF, IHC-P and IP. Anti-c-Myc antibody [Y69] - ChIP Grade (ab32072) was first used in a scientific publication in 1985 and has been cited over 1320 times in peer reviewed journals. It's performance in Western blot and IHC in human and mouse samples is trusted by the scientific community. Abcam's high quality manufacturing and validation processes ensure Anti-c-Myc antibody [Y69] - ChIP Grade (ab32072) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility. The specificity of Anti-c-Myc antibody [Y69] - ChIP Grade (ab32072) has been confirmed by Western Blot testing in c-Myc knockout HEK-293T cells ( ab256500 ). Anti-c-Myc antibody [Y69] - ChIP Grade (ab32072) has 55 independent reviews from customers. c-Myc antibodies are used to visualize proteins labelled with this tag in a variety of applications (for example imaging and Flow cytometry). To enable specific detection of your tagged protein, Anti-c-Myc antibody [Y69] - ChIP Grade (ab32072) has been validated in ab256500 ChIC/CUT&RUN-seq, ChIP,-seq Flow Cyt (Intra), ICC/IF, IHC-P and IP. Anti-c-Myc antibody [Y69] - ChIP Grade (ab32072) specifically detects c-Myc (UniProt ID: P01106; Molecular weight: 51kDa) and is sold in a convenient trial size to enable initial testing (20 µL) and larger sizes for subsequent scaling up experiments (100 µL and 1 mL). Conjugation-ready, carrier free format available for antibody clone Y69 - ab168727 . Antibody clone Y69 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor ® 488, Alexa Fluor ® 647, Alexa Fluor ® 594, Alexa Fluor ® 555, HRP, FITC ( ab190026 , ab190560 , ab201775 , ab201780 , ab205818 , ab223913 ). Only antibody in the market to this target validated for both CUT&RUN and ChIP-seq, which are key applications to map protein-DNA interactions on a genome-wide scale using NGS. Highly-specific antibody targeting c-Myc, a crucial transcription factor involved in various cellular processes including cancer biology, cell cycle regulation and apoptosis studies. Widely used in cancer research, this antibody is crucial for investigating c-Myc's role in tumorigenesis, including Burkitt lymphoma, multiple myeloma and glioblastoma. Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies .

Host

Rabbit

Reactivity

Mouse, Rat, Human

Application

ICC/IF, ChIC/CUT&RUN-seq, IP, ChIP-seq, Flow Cyt (Intra), WB, IHC-P

Platform ID

BAB671400775

Abcam

Headquarters

Discovery Drive Cambridge Biomedical Campus Cambridge CB2 0AX UK

Contact

Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215

Product Specifications
Scientific Background

Specifications

NameAnti-c-Myc antibody [Y69] - ChIP Grade
Cat. No.AB32072
HostRabbit
IsotypeIgG
ReactivityMouse, Rat, Human
ApplicationICC/IF, ChIC/CUT&RUN-seq, IP, ChIP-seq, Flow Cyt (Intra), WB, IHC-P
ClonalityMonoclonal
Clone NumberY69
Concentration0.529 mg/mL Batch dependent concentration
ImmunogenThe exact immunogen used to generate this antibody is proprietary information.
PurityAffinity purification Protein A
Appearance/FormLiquid
ShippingBlue Ice
FormulationpH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Storage-20°C
Regulatory StatusResearch Use Only

Scientific Background

Target data Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3' (PubMed : 24940000, PubMed : 25956029). Activates the transcription of growth-related genes (PubMed : 24940000, PubMed : 25956029). Binds to the VEGFA promoter, promoting VEGFA production and subsequent sprouting angiogenesis (PubMed : 24940000, PubMed : 25956029). Regulator of somatic reprogramming, controls self-renewal of embryonic stem cells (By similarity). Functions with TAF6L to activate target gene expression through RNA polymerase II pause release (By similarity). Positively regulates transcription of HNRNPA1, HNRNPA2 and PTBP1 which in turn regulate splicing of pyruvate kinase PKM by binding repressively to sequences flanking PKM exon 9, inhibiting exon 9 inclusion and resulting in exon 10 inclusion and production of the PKM M2 isoform (PubMed : 20010808). See full target information MYC

Category Paths

Request a product

Please provide the required information below so that we can quickly source your products.