Anti-eRF3/GSPT1 antibody,Abcam,AB49878

What is this antibody validated in? Anti-eRF3/GSPT1 antibody (ab49878) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples. Trusted by the scientific community Anti-eRF3/GSPT1 (ab49878) was first used in a scientific publication in 2007 and has been cited over 10 times in peer-reviewed journals.

Host

Rabbit

Reactivity

Human

Application

IP, WB, ICC/IF

Platform ID

BAB732971207

Abcam

Headquarters

Discovery Drive Cambridge Biomedical Campus Cambridge CB2 0AX UK

Abcam

Contact

Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215

Email:

[email protected]

Product Specifications
Scientific Background

Specifications

NameAnti-eRF3/GSPT1 antibody
Cat. No.AB49878
HostRabbit
IsotypeIgG
ReactivityHuman
ApplicationIP, WB, ICC/IF
ClonalityPolyclonal
Concentration1.3 mg/mL Batch dependent concentration
ImmunogenSynthetic Peptide within Human GSPT1 aa 150-250 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.
PurityAffinity purification Immunogen
Appearance/FormLiquid
ShippingBlue Ice
FormulationpH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS
Storage-20°C
Regulatory StatusResearch Use Only

Scientific Background

Target data GTPase component of the eRF1-eRF3-GTP ternary complex, a ternary complex that mediates translation termination in response to the termination codons UAA, UAG and UGA (PubMed : 15987998, PubMed : 19417105, PubMed : 2511002, PubMed : 27863242). GSPT1/ERF3A mediates ETF1/ERF1 delivery to stop codons : The eRF1-eRF3-GTP complex binds to a stop codon in the ribosomal A-site (PubMed : 27863242). GTP hydrolysis by GSPT1/ERF3A induces a conformational change that leads to its dissociation, permitting ETF1/ERF1 to accommodate fully in the A-site (PubMed : 16777602, PubMed : 27863242). Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons (PubMed : 24486019). Required for SHFL-mediated translation termination which inhibits programmed ribosomal frameshifting (-1PRF) of mRNA from viruses and cellular genes (PubMed : 30682371). See full target information GSPT1

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