Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y],Abcam,AB81299
Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] (ab81299) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Dot Blot, ICC/IF, IHC-P, IP and WB. Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] (ab81299) was first used in a scientific publication in 2008 and has been cited over 272 times in peer reviewed journals. It's performance in Western Blot and immunofluorescence in human samples is trusted by the scientific community. Abcam's high quality manufacturing and validation processes ensure Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] (ab81299) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility. Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] (ab81299) has 16 independent reviews from customers. Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] (ab81299) specifically detects gamma H2A.X Phospho-S139 (UniProt ID: P16104; Molecular weight: 16kDa) and is sold in a convenient trial size to enable initial testing (20 µL) and larger sizes for subsequent scaling up experiments (100 µL and 1 mL). Conjugation-ready, carrier free format available for antibody clone EP854(2)Y - ab215967 . Antibody clone EP854(2)Y is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor ® 488, Alexa Fluor ® 647, HRP, Alexa Fluor ® 594, Alexa Fluor ® 555 ( ab195188 , ab195189 , ab195190 , ab206898 , ab206900 ). Gamma H2A.X (γH2A.X) is a phosphorylated form of the H2A histone family member X (H2AX), which plays a crucial role in the cellular response to DNA damage. When double-strand breaks occur in DNA, H2AX is rapidly phosphorylated at serine 139 to form γH2A.X. This modification serves as a marker for DNA damage and is essential for recruiting DNA repair proteins to the site of damage. Researchers use γH2A.X as a sensitive indicator of DNA double-strand breaks in studies of genotoxic stress, cancer and other conditions involving DNA damage and repair. Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies .
Host
Rabbit
Reactivity
Mouse, Rat, Human
Application
Dot, WB, IP, ICC/IF, IHC-P
Platform ID
BAB439056032

Abcam
Contact
Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215
Email:
Specifications
Scientific Background
Target data The protein expressed by the H2AX gene is a variant histone H2A that replaces conventional H2A in certain nucleosomes, which are responsible for wrapping and compacting DNA into chromatin. This compaction limits DNA accessibility to cellular machineries that require DNA as a template, placing histones at the center of transcription regulation, DNA repair, DNA replication, and chromosomal stability. DNA accessibility is controlled through a complex array of post-translational histone modifications, known as the histone code, and nucleosome remodeling. The H2AX protein is essential for the checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for the efficient repair of DNA double strand breaks (DSBs), particularly when it undergoes C-terminal phosphorylation. This supplementary information is collated from multiple sources and compiled automatically. See full target information H2AX phospho S139
Category Paths
- Products>Primary Antibodies>Monoclonal Antibodies
- Products>Primary Antibodies>IHC Antibodies
- Products>Primary Antibodies>Recombinant Antibodies
- Products>Trial Size Antibodies
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