Anti-gamma H2A.X (phospho S139) antibody [N1-431],Abcam,AB303656

What is this antibody validated in? Anti-gamma H2A.X (phospho S139) antibody [N1-431] (ab303656) is a mouse recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunocytochemistry/immunofluorescence (ICC/IF), Dot Blot, Peptide Array in Human, Mouse samples. What is the molecular weight of gamma H2A.X? Anti-gamma H2A.X (phospho S139) [N1-431] (ab303656) specifically detects a band for gamma H2A.X (UniProt: P16104) at a molecular weight of 15kDa. What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies . Want a custom formulation? This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact [email protected]

Host

Mouse

Reactivity

Human, Mouse

Application

PepArr, WB, ICC/IF, Flow Cyt (Intra), Dot

Platform ID

BAB064068526

Abcam

Headquarters

Discovery Drive Cambridge Biomedical Campus Cambridge CB2 0AX UK

Abcam

Contact

Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215

Email:

[email protected]

Product Specifications
Scientific Background

Specifications

NameAnti-gamma H2A.X (phospho S139) antibody [N1-431]
Cat. No.AB303656
HostMouse
IsotypeIgG1
ReactivityHuman, Mouse
ApplicationPepArr, WB, ICC/IF, Flow Cyt (Intra), Dot
ClonalityMonoclonal
Clone NumberN1- 431
Concentration1.007 mg/mL Batch dependent concentration
ImmunogenThe exact immunogen used to generate this antibody is proprietary information.
PurityAffinity purification Protein A
Appearance/FormLiquid
ShippingBlue Ice
FormulationpH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Storage-20°C
Regulatory StatusResearch Use Only

Scientific Background

Target data Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation. See full target information H2AX

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