Anti-non-muscle Myosin IIB/MYH10 antibody [EPR22564-23] - Rat IgG2a (Chimeric) - BSA and Azide free,Abcam,AB300648

ab300648 is a carrier free version of ab300647 . This rat monoclonal chimeric antibody has been engineered from a RabMAb parent antibody ( ab230823 ). By necessity, some rabbit sequence is retained as part of the variable domain. When multiplexing with other rabbit-derived antibodies, using cross absorbed FC-reactive secondary antibodies are recommended. What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies . Conjugation ready Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Host

Rat

Reactivity

Human, Mouse, Rat

Application

ICC/IF, Flow Cyt (Intra), IHC-P, WB, IP

Platform ID

BAB653875398

Abcam

Headquarters

Discovery Drive Cambridge Biomedical Campus Cambridge CB2 0AX UK

Contact

Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215

Product Specifications
Scientific Background

Specifications

NameAnti-non-muscle Myosin IIB/MYH10 antibody [EPR22564-23] - Rat IgG2a (Chimeric) - BSA and Azide free
Cat. No.AB300648
HostRat
IsotypeIgG2a
ReactivityHuman, Mouse, Rat
ApplicationICC/IF, Flow Cyt (Intra), IHC-P, WB, IP
ClonalityMonoclonal
Clone NumberEPR22564-23
Concentration0.978 mg/mL Batch dependent concentration
ImmunogenThe exact immunogen used to generate this antibody is proprietary information.
PurityIon exchange chromatography DEAE-C
Appearance/FormLiquid
ShippingBlue Ice
FormulationpH: 7.2 - 7.4 Constituents: PBS
Storage+4°C
Regulatory StatusResearch Use Only

Scientific Background

Target data Cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. During cell spreading, plays an important role in cytoskeleton reorganization, focal contacts formation (in the central part but not the margins of spreading cells), and lamellipodial extension; this function is mechanically antagonized by MYH9.. (Microbial infection) Acts as a receptor for herpes simplex virus 1/HHV-1 envelope glycoprotein B. See full target information MYH10

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