Anti-p21 antibody [EPR362],Abcam,AB109520

Anti-p21 antibody [EPR362] (ab109520) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt (Intra), ICC/IF, IHC-P, IP and WB. Anti-p21 antibody [EPR362] (ab109520) was first used in a scientific publication in 2014 and has been cited over 391 times in peer reviewed journals. It's performance in Western Blot in human samples is trusted by the scientific community. Abcam's high quality manufacturing and validation processes ensure Anti-p21 antibody [EPR362] (ab109520) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility. The specificity of Anti-p21 antibody [EPR362] (ab109520) has been confirmed by Western Blot testing in p21 knockout HeLa cells ( ab255349 ). Anti-p21 antibody [EPR362] (ab109520) has 13 independent reviews from customers. Anti-p21 antibody [EPR362] (ab109520) specifically detects p21 (UniProt ID: P38936; Molecular weight: 18kDa) and is sold in a convenient trial size to enable initial testing (20 µL) and larger sizes for subsequent scaling up experiments (100 µL and 1 mL). Conjugation-ready, carrier free format available for antibody clone EPR362 - ab218311 . Antibody clone EPR362 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor ® 488 ( ab282187 ). p21 is a cyclin-dependent kinase inhibitor that regulates cell cycle progression, promoting cell cycle arrest in response to DNA damage. It plays a dual role in oncology by both suppressing tumor growth through cell cycle inhibition and contributing to cancer progression when localized in the cytoplasm. Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies . Shipping conditions update: ambient shipping This product will be delivered at ambient temperature instead of chilled – this is by design. Extensive stability testing confirmed that our products are suitable for shipment under ambient conditions and maintain expected quality. Why the change? It’s part of our commitment to more sustainable packaging solutions, with ambient deliveries using eco-friendly materials such as recyclable cardboard instead of polystyrene. What you need to know Ambient shipments come clearly marked on the delivery note. No ice will be included in ambient shipments, but mixed orders (ambient and cold-chain items) will still arrive with ice packs to protect temperature-sensitive products. Warranty coverage remains fully valid, aligned with our validated shipping method. Please store the product as per the datasheet instructions upon receipt. Find out more - https://www.abcam.com/en-us/support/shipping-storage-support/ambient-shipping

Host

Rabbit

Reactivity

Human

Application

WB, ICC/IF, IHC-P, IP, Flow Cyt (Intra)

Platform ID

BAB785634652

Abcam

Headquarters

Discovery Drive Cambridge Biomedical Campus Cambridge CB2 0AX UK

Contact

Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215

Product Specifications
Scientific Background

Specifications

NameAnti-p21 antibody [EPR362]
Cat. No.AB109520
HostRabbit
IsotypeIgG
ReactivityHuman
ApplicationWB, ICC/IF, IHC-P, IP, Flow Cyt (Intra)
ClonalityMonoclonal
Clone NumberEPR362
Concentration0.817 mg/mL Batch dependent concentration
ImmunogenThe exact immunogen used to generate this antibody is proprietary information.
PurityAffinity purification Protein A
Appearance/FormLiquid
ShippingConditional Ambient
FormulationpH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Storage-20°C
Regulatory StatusResearch Use Only

Scientific Background

Target data Plays an important role in controlling cell cycle progression and DNA damage-induced G2 arrest (PubMed : 9106657). Involved in p53/TP53 mediated inhibition of cellular proliferation in response to DNA damage. Also involved in p53-independent DNA damage-induced G2 arrest mediated by CREB3L1 in astrocytes and osteoblasts (By similarity). Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex. Inhibits DNA synthesis by DNA polymerase delta by competing with POLD3 for PCNA binding (PubMed : 11595739). Negatively regulates the CDK4- and CDK6-driven phosphorylation of RB1 in keratinocytes, thereby resulting in the release of E2F1 and subsequent transcription of E2F1-driven G1/S phase promoting genes (By similarity). See full target information CDKN1A

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