Anti-p27 KIP 1 antibody [Y236],Abcam,AB32034

What is this antibody validated in? Anti-p27 KIP 1 antibody [Y236] (ab32034) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples. What is the molecular weight of p27 KIP 1? Anti-p27 KIP 1 [Y236] (ab32034) specifically detects a band for p27 KIP 1 (UniProt: P46527) at a molecular weight of 22kDa. Trusted by the scientific community Anti-p27 KIP 1 [Y236] (ab32034) was first used in a scientific publication in 2006 and has been cited over 200 times in peer-reviewed journals. Reviewed by scientists Anti-p27 KIP 1 [Y236] (ab32034) has over 15 independent reviews from customers. Trial sizes available! Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies. Specificity confirmed The specificity of Anti-p27 KIP 1 antibody [Y236] (ab32034) has been confirmed by Western blot testing in CDKN1B Knockout HAP1 cells. Other related products We have a range of other formats of antibody clone [Y236] also available for your convenience: ab32034, HRP - ab194235 , Carrier free - ab206927 , APC - ab310850 , PE - ab310920 Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies .

Host

Rabbit

Reactivity

Mouse, Rat, Human

Application

IP, WB, ICC/IF, IHC-P, Flow Cyt (Intra)

Platform ID

BAB223303321

Abcam

Headquarters

Discovery Drive Cambridge Biomedical Campus Cambridge CB2 0AX UK

Contact

Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215

Product Specifications
Scientific Background

Specifications

NameAnti-p27 KIP 1 antibody [Y236]
Cat. No.AB32034
HostRabbit
IsotypeIgG
ReactivityMouse, Rat, Human
ApplicationIP, WB, ICC/IF, IHC-P, Flow Cyt (Intra)
ClonalityMonoclonal
Clone NumberY236
Concentration0.566 mg/mL Batch dependent concentration
ImmunogenThe exact immunogen used to generate this antibody is proprietary information.
PurityAffinity purification Protein A
Appearance/FormLiquid
ShippingBlue Ice
FormulationpH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Storage-20°C
Regulatory StatusResearch Use Only

Scientific Background

Target data Important regulator of cell cycle progression. Inhibits the kinase activity of CDK2 bound to cyclin A, but has little inhibitory activity on CDK2 bound to SPDYA (PubMed : 28666995). Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry. See full target information CDKN1B

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