Anti-xCT antibody [EPR27115-64],Abcam,AB307601
Anti-xCT antibody [EPR27115-64] (ab307601) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt (Intra), ICC/IF, IHC-P, IP and WB. Abcam's high quality manufacturing and validation processes ensure Anti-xCT antibody [EPR27115-64] (ab307601) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility. Anti-xCT antibody [EPR27115-64] (ab307601) specifically detects xCT (UniProt ID: Q9UPY5; Molecular weight: 56kDa) and is sold in a convenient trial size to enable initial testing (20 µL) and larger sizes for subsequent scaling up experiments (100 µL and 1 mL). Conjugation-ready, carrier free format available for antibody clone EPR27115-64 - ab307602 . A highly validated xCT (SLC7A11) antibody, essential for studying the cystine/glutamate antiporter SLC7A11, ferroptosis and redox balance. This antibody is crucial in cancer research, particularly in understanding xCT's role in tumor progression and antioxidant defense and is widely used in studies of reactive oxygen species (ROS) and xCT inhibitors for therapeutic applications. SLC7A11 is frequently overexpressed in human cancers. SLC7A11 promotes cystine uptake and glutathione biosynthesis, resulting in protection from oxidative stress and ferroptotic cell death. Recent studies have unexpectedly revealed that SLC7A11 also plays critical roles in glutamine metabolism and regulates the glucose and glutamine dependency of cancer cells. Expression of SLC7A11/xCT correlates with advanced lung cancer stage and predicted a worse 5-year survival. Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies .
Host
Rabbit
Reactivity
Rat, Mouse, Human
Application
Flow Cyt (Intra), IHC-P, IP, ICC/IF, WB
Platform ID
BAB578177957
Abcam
Contact
Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215
Email:
Specifications
Scientific Background
Target data Heterodimer with SLC3A2, that functions as an antiporter by mediating the exchange of extracellular anionic L-cystine and intracellular L-glutamate across the cellular plasma membrane (PubMed : 11133847, PubMed : 11417227, PubMed : 14722095, PubMed : 15151999, PubMed : 34880232, PubMed : 35245456, PubMed : 35352032). Provides L-cystine for the maintenance of the redox balance between extracellular L-cystine and L-cysteine and for the maintenance of the intracellular levels of glutathione that is essential for cells protection from oxidative stress (By similarity). The transport is sodium-independent, electroneutral with a stoichiometry of 1 : 1, and is drove by the high intracellular concentration of L-glutamate and the intracellular reduction of L-cystine (PubMed : 11133847, PubMed : 11417227). Acts as an inhibitor of ferroptosis by mediating the import of L-kynurenine leading to anti-ferroptotic signaling propagation required to maintain L-cystine and glutathione homeostasis (PubMed : 35245456, PubMed : 40246981). Also inhibits ferroptosis by acting as an atypical proton transporter that mediates a slow proton efflux from lysosomes via cystine and glutamate flux (PubMed : 40280132). Glutamate and cystine contain side-chain groups that are protonatable in the physiological range of lysosomal pH and cytosolic pH, respectively, enabling a slow lysosomal proton leak through a substrate-as-proton mechanism (PubMed : 40280132). Moreover, mediates N-acetyl-L-cysteine uptake into the placenta leading to subsequently down-regulation of pathways associated with oxidative stress, inflammation and apoptosis (PubMed : 34120018). In vitro can also transport L-aspartate (PubMed : 11417227). May participate in astrocyte and meningeal cell proliferation during development and can provide neuroprotection by promoting glutathione synthesis and delivery from non-neuronal cells such as astrocytes and meningeal cells to immature neurons (By similarity). Controls the production of pheomelanin pigment directly (By similarity). See full target information Cystine/glutamate transporter
Category Paths
- Products>Primary Antibodies>Monoclonal Antibodies
- Products>Primary Antibodies>IHC Antibodies
- Products>Primary Antibodies>Recombinant Antibodies
- Products>Trial Size Antibodies
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