Atg2A Antibody#15011,Cell Signaling Technology (CST),15011

Autophagy is a catabolic process that results in the degradation of bulk cytoplasmic contents within autophagosomes and lysosomes. The control of autophagy involves proteins encoded by a set of autophagy-related genes (Atg) that were originally characterized in yeast (1). Research studies in yeast indicate that Atg2 is essential for autophagy and the retrograde transport of Atg9 through an interaction with Atg18 (2-6). Two human Atg2 homologs (Atg2A, Atg2B) are critical for autophagosome formation as silencing of both results in the accumulation of unclosed autophagic structures (7). Starvation-induced autophagy targets Atg2A to the initiation site of autophagosome biogenesis, where it associates with DFCP1, WIPI-1, and other autophagy-related proteins (8).Atg2 proteins also function in lipid droplet metabolism as depletion of both Atg2A and AtgB results in changes in the size, number, and distribution of lipid droplets (7,8). These morphological changes in lipid droplets are not observed in Atg5-depleted cells, suggesting that this function is independent of the role of Atg2 in autophagy (7). Starvation-induced autophagy directs Atg2A (along with Atg14L) to localize to early autophagosomal membranes enriched in PI3P, while another subpopulation of Atg2A and Atg14L localizes to the lipid droplets independent of autophagic status (8). An increase in Atg2A expression during etoposide- and doxorubicin-induced apoptosis suggests that Atg2A may be a useful indicator of topoisomerase II inhibitor-mediated apoptosis (9). Mutations in the correspondingAtg2Bgene are associated with gastric and colorectal carcinomas with high microsatellite instability (10).Reggiori, F. and Klionsky, D.J. (2002)Eukaryot Cell1, 11-21.Shintani, T. et al. (2001)J Biol Chem276, 30452-60.Wang, C.W. et al. (2001)J Biol Chem276, 30442-51.Suzuki, K. et al. (2001)EMBO J20, 5971-81.Obara, K. et al. (2008)J Biol Chem283, 23972-80.Reggiori, F. et al. (2004)Dev Cell6, 79-90.Velikkakath, A.K. et al. (2012)Mol Biol Cell23, 896-909.Pfisterer, S.G. et al. (2014)J Lipid Res55, 1267-78.Kusama, Y. et al. (2009)Apoptosis14, 1165-75.Kang, M.R. et al. (2009)J Pathol217, 702-6.Alternate NamesATG2 autophagy related 2 homolog A; ATG2 autophagy related 2 homolog A (S. cerevisiae); ATG2A; autophagy related 2A; Autophagy-related protein 2 homolog A; KIAA0404; MGC117153

ATG2 autophagy related 2 homolog A; ATG2 autophagy related 2 homolog A (S. cerevisiae); ATG2A; autophagy related 2A; Autophagy-related protein 2 homolog A; KIAA0404; MGC117153