BRD9 (E4Q3F) Rabbit Monoclonal Antibody (SignalFlexTMAlexa Fluor®647 Conjugate)#10631,Cell Signaling Technology (CST),10631

BRD9 (E4Q3F) Rabbit mAb (SignalFlexTM Alexa Fluor®647 Conjugate) recognizes endogenous levels of total BRD9 protein. This antibody does not cross-react with BRD7 protein.

Host

Rabbit

Reactivity

Human

Conjugate

SignalFlexTMAlexa Fluor®647 Conjugate

Platform ID

BAB066312506

Cell Signaling Technology (CST)

Headquarters

3 Trask Lane Danvers, MA 01923

Cell Signaling Technology (CST)

Contact

Tel: 877-616-2355,978-867-2388
Fax: 877-616-2355

Email:

[email protected]

Product Specifications
Scientific Background
Synonyms

Specifications

NameBRD9 (E4Q3F) Rabbit Monoclonal Antibody (SignalFlexTMAlexa Fluor®647 Conjugate)#10631
Cat. No.10631
Accession NumberQ9H8M2
Gene ID (Entrez)65980
HostRabbit
SensitivityEndogenous
ReactivityHuman
ConjugationSignalFlexTMAlexa Fluor®647 Conjugate
ImmunogenIgG
FormulationSignalFlexTM conjugates are produced using highly validated Cell Signaling Technology®primary antibodies and conjugation methods that have been rigorously tested, ensuring high-quality conjugates and lot-to-lot consistency. These conjugates are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. However, they are not tested on specific assays.Optimal dilutions/concentrations should be determined by the end user. When performing flow cytometry, we recommend using an isotype control conjugate at the same concentration as the antibody conjugate.
StorageSupplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C.Do not aliquot the antibody. Protect from light. Do not freeze.
Regulatory StatusResearch Use Only

Scientific Background

ATP-dependent chromatin remodeling complexes play an essential role in the regulation of various nuclear processes, such as gene expression, DNA replication, and repair (1,2). The SWI/SNF chromatin remodeling complex consists of more than 10 subunits with a single molecule of the ATPase catalytic subunit BRM or BRG1, but not both. The activities of these two subunits drive the disruption of histone-DNA contacts that lead to changes in accessibility of crucial regulatory elements within chromatin (2-5). The BRM/BRG1 containing SWI/SNF complexes are recruited to target promoters by transcription factors, such as nuclear receptors, p53, RB, and BRCA1, to regulate gene activation, cell growth, the cell cycle, and differentiation processes (1,6-9).GLTSCR1 and its paralog GLTSCR1L, along with BRD9, have been identified as unique subunits of the non-canonical BAF (ncBAF) or GBAF complex. This complex contains either GLTSCR1 or GLTSCR1L instead of an ARID subunit, while also lacking the BAF45, BAF47, and BAF57 subunits. GBAF maps to regions distinct from the PBAF and canonical BAF complexes and has also been shown to be a synthetic lethal target for BAF-driven cancers, such as synovial sarcomas and rhabdoid tumors (10-12).Ho, L. and Crabtree, G.R. (2010)Nature463, 474-84.Becker, P.B. and Hörz, W. (2002)Annu Rev Biochem71, 247-73.Eberharter, A. and Becker, P.B. (2004)J Cell Sci117, 3707-11.Bowman, G.D. (2010)Curr Opin Struct Biol20, 73-81.Gangaraju, V.K. and Bartholomew, B. (2007)Mutat Res618, 3-17.Lessard, J.A. and Crabtree, G.R. (2010)Annu Rev Cell Dev Biol26, 503-32.Morettini, S. et al. (2008)Front Biosci13, 5522-32.Wolf, I.M. et al. (2008)J Cell Biochem104, 1580-6.Simone, C. (2006)J Cell Physiol207, 309-14.Alpsoy, A. and Dykhuizen, E.C. (2018)J Biol Chem293, 3892-903.Mashtalir, N. et al. (2018)Cell175, 1272-1288.e20.Michel, B.C. et al. (2018)Nat Cell Biol20, 1410-20.Alternate NamesBRD9; bromodomain containing 9; Bromodomain-containing protein 9; DKFZp434D0711; DKFZp686L0539; FLJ43530; LAVS3040; PRO9856; Rhabdomyosarcoma antigen MU-RMS-40.8; sarcoma antigen NY-SAR-29

Synonyms

BRD9; bromodomain containing 9; Bromodomain-containing protein 9; DKFZp434D0711; DKFZp686L0539; FLJ43530; LAVS3040; PRO9856; Rhabdomyosarcoma antigen MU-RMS-40.8; sarcoma antigen NY-SAR-29

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