Beta-Actin Mouse Monoclonal Antibody (BA3R),Medimabs,MM-0164-P

Host

Mouse

Reactivity

Chicken, Human, Mouse, rabbit, Rat

Application

Dot, ELISA, IHC, WB

Platform ID

BAB711902474

Medimabs

Headquarters

141 avenue du President-Kennedy 6th floor, Suite SB-6570 Montreal (Quebec) Canada, H2X 1Y4

Medimabs

Contact

Tel: 1 (514) 940-MABS (6227)
Fax: 1 (514) 733-1212

Email:

[email protected]

Product Specifications
Scientific Background

Specifications

NameBeta-Actin Mouse Monoclonal Antibody (BA3R)
Cat. No.MM-0164-P
HostMouse
IsotypeIgG2b
SensitivityThe antibody recognizes native and denatured forms of β-Actin (~42kDa)
ReactivityChicken, Human, Mouse, rabbit, Rat
ApplicationDot, ELISA, IHC, WB
Working DilutionsIf reconstituted with deionized water in 100 µl: WB 1:1000-3,000, IHC 1:500-2,000. Optimal dilution has to be determined by the user.
ClonalityMonoclonal
Clone NumberBA3R
TargetBeta-Actin
Appearance/FormPurified with protein G, stored in PBS pH 7.4 and lyophilized.
StorageLyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.
Regulatory StatusResearch Use Only

Scientific Background

β-actin (gene name ACTB) is one of six different actin isoforms which have been identified in humans. This is one of the two non-muscle cytoskeletal actins. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. The β-actin gene is often stably and constitutively expressed at high levels in most tissues and cells. Proteins such as β-actin, involved in maintenance of basic cellular function, are often referred to as housekeeping proteins. Housekeeping proteins are frequently used as loading controls for western blots and protein normalization.

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