Biotin anti-human CD4 Antibody anti-CD4 - OKT4,BioLegend,317406
The OKT4 antibody binds to the D3 domain of CD4 and does not block HIV binding. Additional reported applications (for the relevant formats) include: immunohistochemistry of frozen sections and blocking of T cell activation. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 317453 and 317454).In a small subset of individuals, the OKT4 clone does not bind to CD4 due to polymorphisms in CD4.9
Host
Mouse
Reactivity
Human, Cynomolgus, Rhesus
Application
FC - Quality tested
Platform ID
BAB811012580
BioLegend
Contact
Tel: 1-858-455-9588
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Product Specifications
Scientific Background
Specifications
NameBiotin anti-human CD4 Antibody anti-CD4 - OKT4
Cat. No.317406
HostMouse
RRIDAB_571948 (BioLegend Cat. No. 317405)AB_571949 (BioLegend Cat. No. 317406)
IsotypeMouse IgG2b, κ
ReactivityHuman, Cynomolgus, Rhesus
ApplicationFC - Quality tested
ClonalityMonoclonal
Clone NumberOKT4
Concentration0.5 mg/ml
TargetCD4
ImmunogenHuman peripheral T cells
PurityThe antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C.Do not freeze.
Regulatory StatusResearch Use Only
Scientific Background
CD4, also known as T4, is a 55 kD single-chain type I transmembrane glycoprotein expressed on most thymocytes, a subset of T cells, and monocytes/macrophages. CD4, a member of the Ig superfamily, recognizes antigens associated with MHC class II molecules and participates in cell-cell interactions, thymic differentiation, and signal transduction. CD4 acts as a primary receptor for HIV, binding to HIV gp120. CD4 has also been shown to interact with IL-16.
Category Paths
- Products>Primary Antibodies>Monoclonal Antibodies
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