Biotin anti-human IL-4 Antibody anti-IL-4 - MP4-25D2,BioLegend,500803
ELISA Capture or ELISpot Capture: The MP4-25D2 antibody is useful as a capture antibody for a sandwich ELISA or ELISPOT assay.Flow Cytometry6,9:The fluorochrome-labeled MP4-25D2 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-4 -producing cells within mixed cell populations.Neutralization1-3:The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of human IL-4 bioactivity (Cat. No. 500815). The MP4-25D2 antibody can neutralize the bioactivity of natural or recombinant IL-4.
Host
Rat
Reactivity
Human
Application
ELISA Detection, ELISPOT Detection, ICFC
Platform ID
BAB443932541
BioLegend
Contact
Tel: 1-858-455-9588
Fax: +49 (4131) 7023913
Email:
Product Specifications
Scientific Background
Specifications
NameBiotin anti-human IL-4 Antibody anti-IL-4 - MP4-25D2
Cat. No.500803
HostRat
RRIDAB_315122 (BioLegend Cat. No. 500803)AB_315123 (BioLegend Cat. No. 500804)
IsotypeRat IgG1, κ
ReactivityHuman
ApplicationELISA Detection, ELISPOT Detection, ICFC
ClonalityMonoclonal
Clone NumberMP4-25D2
Concentration0.5 mg/ml
TargetIL-4
ImmunogenCHO-expressed, recombinant human IL-4
PurityThe antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C.Do not freeze.
Regulatory StatusResearch Use Only
Scientific Background
IL-4 is a pleiotropic cytokine that is produced by activated T cells, mast cells, and basophils. IL-4 elicits many different biological responses but has two dominant functions. The first is regulating differentiation of naïve CD4+T cell to the Th2 type. Th2 cells produce IL-4, IL-5, IL-10, and IL-13, which tend to favor a humoral immune response while suppressing a cell-mediated immune response controlled by Th1 cells. The second is regulating IgE and IgG1 production by B cells.
Category Paths
- Products>Primary Antibodies>Monoclonal Antibodies
Request a product
Please provide the required information below so that we can quickly source your products.