Biotin anti-human MCP-1 Antibody anti-CCL2 - 5D3-F7,BioLegend,502609
ELISA or ELISPOT Detection1:The biotinylated 5D3-F7 antibody is useful as the detection antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the purified 2H5 antibody (Cat. No. 505902/505906) as the capture antibody.ELISA or ELISPOT Capture:The purified 5D3-F7 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated 2H5 antibody (Cat. No. 505908) as the detection antibody. The LEAF™ purified antibody (Cat. No. 502607) is suggested for ELISPOT capture.Additional reported applications (for the relevant formats) include: intracellular flow cytometry2, immunoprecipitation1,3, Western blotting1, and immunohistochemical staining1.
Host
Mouse
Reactivity
Human
Application
ELISA Detection, ELISPOT Detection, ICFC
Platform ID
BAB129138657
BioLegend
Contact
Tel: 1-858-455-9588
Fax: +49 (4131) 7023913
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Product Specifications
Scientific Background
Specifications
NameBiotin anti-human MCP-1 Antibody anti-CCL2 - 5D3-F7
Cat. No.502609
HostMouse
RRIDAB_315247 (BioLegend Cat. No. 502608)AB_315248 (BioLegend Cat. No. 502609)
IsotypeMouse IgG1, κ
ReactivityHuman
ApplicationELISA Detection, ELISPOT Detection, ICFC
ClonalityMonoclonal
Clone Number5D3-F7
Concentration0.5 mg/ml
TargetCCL2
ImmunogenRecombinant human MCP-1
PurityThe antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C.Do not freeze.
Regulatory StatusResearch Use Only
Scientific Background
Monocyte chemotactic protein-1 (MCP-1) also known as monocyte chemotactic and activating factor (MCAF) was identified based on its ability to chemoattract monocytes. Subsequently, MCP-1 has also been found to regulate adhesion molecule expression and cytokine production in monocytes. MCP-1 is identical to the product of the JE gene, a PDGF inducible gene. MCP-1 is a member of the beta (C-C) chemokine subfamily, known as CCL2. The 5D3-F7 antibody reacts with human monocyte chemoattractant protein-1 (MCP-1).
Category Paths
- Products>Primary Antibodies>Monoclonal Antibodies
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