Biotin anti-mouse CD34 Antibody anti-CD34 - MEC14.7,BioLegend,119303

The MEC14.7 antibody does not stain bone marrow cells like some other mouse CD34 antibodies, probably because the antibody recognizes a different epitope from other mAbs. Additional reported applications (for the relevant formats) include: immunoprecipitation, Western blotting6, and immunohistochemistry of acetone-fixed frozen sections and paraffin-embedded sections2,4,5,6.

Host

Rat

Reactivity

Mouse

Application

FC -Quality testedIHC-F -Verified

Platform ID

BAB189609617

BioLegend

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Contact

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Product Specifications
Scientific Background

Specifications

NameBiotin anti-mouse CD34 Antibody anti-CD34 - MEC14.7
Cat. No.119303
HostRat
RRIDAB_345281 (BioLegend Cat. No. 119303)AB_345282 (BioLegend Cat. No. 119304)
IsotypeRat IgG2a, κ
ReactivityMouse
ApplicationFC -Quality testedIHC-F -Verified
ClonalityMonoclonal
Clone NumberMEC14.7
Concentration0.5 mg/ml
TargetCD34
ImmunogenCells transfected with mouse CD34
PurityThe antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C.Do not freeze.
Regulatory StatusResearch Use Only

Scientific Background

CD34 is a highly glycosylated hematopoietic progenitor antigen. Two isoforms of CD34 have been reported to be generated by alternative splicing. This antigen is expressed on hematopoietic progenitors as well as on endothelial cells, brain, and testis. CD34 is thought to function as an adhesion molecule for early hematopoietic progenitors mediating the attachment of stem cells to extracellular matrix or stromal cells. CD34 is phosphorylated on serine residues by PKC.

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