Brilliant Violet 421 anti-human CD8a Antibody anti-CD8a - RPA-T8,BioLegend,301036

The RPA-T8 antibody does not block the binding of HIT8a antibody to CD8a. Additional reported applications of this antibody (for the relevant formats) include: immunohistochemical staining of paraformaldehyde-fixed frozen sections3and costimulation of T cell responses4. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 301073 & 301074).

Host

Mouse

Reactivity

Human, Cynomolgus, Rhesus

Application

FC - Quality testedICC - Verified

Platform ID

BAB212998385

BioLegend

Headquarters

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Contact

Tel: 1-858-455-9588
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Product Specifications
Scientific Background

Specifications

NameBrilliant Violet 421 anti-human CD8a Antibody anti-CD8a - RPA-T8
Cat. No.301036
HostMouse
RRIDAB_10898322 (BioLegend Cat. No. 301035)AB_10960142 (BioLegend Cat. No. 301036)
IsotypeMouse IgG1, κ
ReactivityHuman, Cynomolgus, Rhesus
ApplicationFC - Quality testedICC - Verified
ClonalityMonoclonal
Clone NumberRPA-T8
ConcentrationLot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.)
TargetCD8alpha
PurityThe antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
StorageThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light.Do not freeze.
Regulatory StatusResearch Use Only

Scientific Background

CD8a is a 32-34 kD type I glycoprotein. It forms a homodimer (CD8a/a) or heterodimer (CD8a/b) with CD8b. CD8, also known as T8 and Leu2, is a member of the immunoglobulin superfamily found on the majority of thymocytes, a subset of peripheral blood T cells, and NK cells (which express almost exclusively CD8a homodimers). CD8 acts as a co-receptor with MHC class I-restricted T cell receptors in antigen recognition and T cell activation, and has been shown to play a role in thymic differentiation. Two domains in CD8a are important for function: the extracellular IgSF domain binds the α3domain of MHC class I and the cytoplasmic CXCP motif binds the tyrosine kinase p56 Lck.

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