Brilliant Violet 421 anti-mouse CD197 CCR7 Antibody anti-CD197 - 4B12,BioLegend,120120

The 4B12 antibody does not inhibit binding of ligand to receptor. Additional reported applications (for the relevant formats) include: immunoprecipitation. To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16/CD32, clone 93 (Cat. No. 101301/101302) is recommended prior to immunofluorescent staining.Staining with clone 4B12 is recommended at 37°C(see supplemental data of PE staining at differing temperatures).

Host

Rat

Reactivity

Mouse

Application

FC - Quality tested

Platform ID

BAB685572820

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Product Specifications
Scientific Background

Specifications

NameBrilliant Violet 421 anti-mouse CD197 CCR7 Antibody anti-CD197 - 4B12
Cat. No.120120
HostRat
RRIDAB_10897811 (BioLegend Cat. No. 120119)AB_2561446 (BioLegend Cat. No. 120120)
IsotypeRat IgG2a, κ
ReactivityMouse
ApplicationFC - Quality tested
ClonalityMonoclonal
Clone Number4B12
ConcentrationLot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.)
TargetCD197
ImmunogenMouse CCR7 transfected RBL-2H3 cells
PurityThe antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
StorageThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light.Do not freeze.
Regulatory StatusResearch Use Only

Scientific Background

CD197 is also known as C-C chemokine receptor 7 (CCR7) or EBI-1. CCR7 is a G-coupled rhodopsin-like member of the GPCR superfamily with a predicted molecular weight of 43 kD that is expressed on hematopoietic stem cells, most naive T cells, some memory T cells, B subset, and mature dendritic cells. CCR7 is a receptor for the chemokines CCL19 (MIP3 beta) and SLC (6CKine, Exodus-2, TCA-4, CCL21) that plays a role in thymocytes development, T cell adhesion at intestinal sites, the homeostatic recirculation of memory T cells, and chemotaxis.

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