Brilliant Violet 421 anti-mouse CD279 PD-1 Antibody anti-CD279 - 29F.1A12,BioLegend,135221

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue3,in vivoblocking of PD-1 binding to its ligands2,3, and spatial biology (IBEX)5,6.

Host

Rat

Reactivity

Mouse

Application

FC - Quality testedSB - Reported in the literature, not verified in house

Platform ID

BAB175327740

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Product Specifications
Scientific Background

Specifications

NameBrilliant Violet 421 anti-mouse CD279 PD-1 Antibody anti-CD279 - 29F.1A12
Cat. No.135221
HostRat
RRIDAB_10900085 (BioLegend Cat. No. 135217)AB_2562568 (BioLegend Cat. No. 135221)AB_2561447 (BioLegend Cat. No. 135218)
IsotypeRat IgG2a, κ
ReactivityMouse
ApplicationFC - Quality testedSB - Reported in the literature, not verified in house
ClonalityMonoclonal
Clone Number29F.1A12
Concentrationµg sizes: 0.2 mg/mLµL sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.)
TargetCD279
ImmunogenPD-1 cDNA followed by PD-1-Ig fusion protein
PurityThe antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
StorageThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light.Do not freeze.
Regulatory StatusResearch Use Only

Scientific Background

CD279, also known as programmed death-1 (PD-1), is a 50-55 kD glycoprotein belonging to the CD28 family of the Ig superfamily. PD-1 is expressed on activated splenic T and B cells and thymocytes. It is induced on activated myeloid cells as well. PD-1 is involved in lymphocyte clonal selection and peripheral tolerance through binding its ligands, B7-H1 (PD-L1) and B7-DC (PD-L2). It has been reported that PD-1 and PD-L1 interactions are critical to positive selection and play a role in shaping the T cell repertoire. PD-L1 negative costimulation is essential for prolonged survival of intratesticular islet allografts.

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