Brilliant Violet 421™ anti-mouse CD4 Antibody, anti-mouse CD4, RM4-5,BioLegend,100544
The RM4-5 antibody blocks the binding of GK1.5 antibody and H129.19 antibody to CD4+T cells, but not RM4-4 antibody. Additional reported applications (for the relevant formats) include: blocking of ligand binding,in vivodepletion of CD4+cells1, and immunohistochemistry of acetone-fixed frozen tissue sections2,3,11and paraffin-embedded sections11. Clone RM4-5 is not recommended for immunohistochemistry of formalin-fixed paraffin sections. Instead, acetone frozen or zinc-fixed paraffin sections are recommended. The Ultra-LEAF™ Purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No.100575 and 100576).
Host
Rat
Reactivity
Mouse
Application
FC - Quality testedICC, IHC-F - Verified
Platform ID
BAB085936875
BioLegend
Contact
Tel: 1-858-455-9588
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Product Specifications
Scientific Background
Specifications
NameBrilliant Violet 421™ anti-mouse CD4 Antibody, anti-mouse CD4, RM4-5
Cat. No.100544
HostRat
RRIDAB_10898318 (BioLegend Cat. No. 100543)AB_2563052 (BioLegend Cat. No. 100563)AB_11219790 (BioLegend Cat. No. 100544)
IsotypeRat IgG2a, κ
ReactivityMouse
ApplicationFC - Quality testedICC, IHC-F - Verified
ClonalityMonoclonal
Clone NumberRM4-5
Concentrationµg sizes: 0.2 mg/mLµL sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.)
TargetCD4
ImmunogenBALB/c mouse thymocytes
PurityThe antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
StorageThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light.Do not freeze.
Regulatory StatusResearch Use Only
Scientific Background
CD4 is a 55 kD protein also known as L3T4 or T4. It is a member of the Ig superfamily, primarily expressed on most thymocytes and a subset of T cells, and weakly on macrophages and dendritic cells. It acts as a co-receptor with the TCR during T cell activation and thymic differentiation by binding MHC class II and associating with the protein tyrosine kinase lck.
Category Paths
- Products>Primary Antibodies>IHC Antibodies
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