Brilliant Violet 421 anti-mouse CD45.2 Antibody anti-CD45.2 - 104,BioLegend,109831

The 104 antibody does not react with mouse cells expressing the CD45.1 alloantigen. Additional reported applications (for the relevant formats) include: immunoprecipitation4,in vivoandin vitroblocking of B cell responses1,2, and immunohistochemical staining of acetone-fixed frozen sections3.

Host

Mouse

Reactivity

Mouse

Application

FC - Quality tested

Platform ID

BAB286796738

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Product Specifications
Scientific Background

Specifications

NameBrilliant Violet 421 anti-mouse CD45.2 Antibody anti-CD45.2 - 104
Cat. No.109831
HostMouse
RRIDAB_10900256 (BioLegend Cat. No. 109831)AB_2565511 (BioLegend Cat. No. 109832)
IsotypeMouse (SJL) IgG2a, κ
ReactivityMouse
ApplicationFC - Quality tested
ClonalityMonoclonal
Clone Number104
Concentrationµg sizes: 0.2 mg/mLµL sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.)
TargetCD45.2
ImmunogenB10.S mouse thymocytes and splenocytes
PurityThe antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
StorageThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light.Do not freeze.
Regulatory StatusResearch Use Only

Scientific Background

CD45.2 is an alloantigen of CD45, expressed by Ly5.2 bearing mouse strains (e.g., A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, 129). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in the mouse that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

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