Brilliant Violet 421 anti-mouse IL-4 Antibody anti-IL-4 - 11B11,BioLegend,504120
ELISA1,2,10,13or ELISPOT5Capture:The purified 11B11 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated BVD6-24G2 antibody (Cat. No. 504202) as the detecting antibody and recombinant mouse IL-4 (Cat. No. 575609) as the standard. The LEAF™ purified antibody is suggested for ELISPOT capture.Neutralization1-2,9,12:The 11B11 antibody can neutralize the bioactivity of natural or recombinant IL-4. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of mouse IL-4 bioactivityin vivoandin vitro(Cat. No. 504108).Additional reported applications (for the relevant formats) include:immunoprecipitation16, immunohistochemical staining of formalin-fixed paraffin-embedded tissue sections8and paraformaldehyde-fixed, saponin-treated frozen tissue sections6,7, and immunocytochemistry4.Note:For testing mouse IL-4 in serum, plasma or supernatant, BioLegend's ELISA Max™ Sets (Cat. No. 431101 to 431106) are specially developed and recommended.
Host
Rat
Reactivity
Mouse
Application
ICFC - Quality tested
Platform ID
BAB788945706
BioLegend
Contact
Tel: 1-858-455-9588
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Product Specifications
Scientific Background
Specifications
NameBrilliant Violet 421 anti-mouse IL-4 Antibody anti-IL-4 - 11B11
Cat. No.504120
HostRat
RRIDAB_10896945 (BioLegend Cat. No. 504119)AB_2562594 (BioLegend Cat. No. 504127)AB_2562102 (BioLegend Cat. No. 504120)
IsotypeRat IgG1, κ
ReactivityMouse
ApplicationICFC - Quality tested
ClonalityMonoclonal
Clone Number11B11
Concentrationµg sizes: 0.2 mg/mLµL sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.)
TargetIL-4
ImmunogenPartially purified native mouse IL-4
PurityThe antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
StorageThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light.Do not freeze.
Regulatory StatusResearch Use Only
Scientific Background
IL-4 is a pleiotropic cytokine produced by activated T cells, mast cells, and basophils. IL-4 is a potent lymphoid cell growth factor which stimulates the growth and activation of certain B cells and T cells. IL-4 is important for regulation of T helper subset development.
Category Paths
- Products>Primary Antibodies>Monoclonal Antibodies
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