Brilliant Violet 510 anti-human CD4 Antibody anti-CD4 - OKT4,BioLegend,317444
The OKT4 antibody binds to the D3 domain of CD4 and does not block HIV binding. Additional reported applications (for the relevant formats) include: immunohistochemistry of frozen sections and blocking of T cell activation. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 317453 and 317454).In a small subset of individuals, the OKT4 clone does not bind to CD4 due to polymorphisms in CD4.9
Host
Mouse
Reactivity
Human, Cynomolgus, Rhesus
Application
FC - Quality tested
Platform ID
BAB998756213
BioLegend
Contact
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Product Specifications
Scientific Background
Specifications
NameBrilliant Violet 510 anti-human CD4 Antibody anti-CD4 - OKT4
Cat. No.317444
HostMouse
RRIDAB_2561377 (BioLegend Cat. No. 317443)AB_2561866 (BioLegend Cat. No. 317444)
IsotypeMouse IgG2b, κ
ReactivityHuman, Cynomolgus, Rhesus
ApplicationFC - Quality tested
ClonalityMonoclonal
Clone NumberOKT4
ConcentrationLot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.)
TargetCD4
ImmunogenHuman peripheral T cells
PurityThe antibody was purified by affinity chromatography and conjugated with Brilliant Violet 510™ under optimal conditions.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
StorageThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light.Do not freeze.
Regulatory StatusResearch Use Only
Scientific Background
CD4, also known as T4, is a 55 kD single-chain type I transmembrane glycoprotein expressed on most thymocytes, a subset of T cells, and monocytes/macrophages. CD4, a member of the Ig superfamily, recognizes antigens associated with MHC class II molecules and participates in cell-cell interactions, thymic differentiation, and signal transduction. CD4 acts as a primary receptor for HIV, binding to HIV gp120. CD4 has also been shown to interact with IL-16.
Category Paths
- Products>Primary Antibodies>Monoclonal Antibodies
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