Brilliant Violet 605™ anti-human CD8a Antibody, CD8a, HIT8a,BioLegend,300936

Clone HIT8a recognizes the alpha chain of CD85. It does not block the binding of RPA-T8 antibody to CD8a.Additional reported applications of this antibody (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections5,6. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue.

Host

Mouse

Reactivity

Human

Application

FC -Quality tested

Platform ID

BAB547005571

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Product Specifications
Scientific Background

Specifications

NameBrilliant Violet 605™ anti-human CD8a Antibody, CD8a, HIT8a
Cat. No.300936
HostMouse
RRIDAB_2832574 (BioLegend Cat. No. 300935)AB_2832575 (BioLegend Cat. No. 300936)
IsotypeMouse IgG1, κ
ReactivityHuman
ApplicationFC -Quality tested
ClonalityMonoclonal
Clone NumberHIT8a
ConcentrationLot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.)
TargetCD8alpha
PurityThe antibody was purified by affinity chromatography and conjugated with Brilliant Violet 605™ under optimal conditions.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
StorageThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light.Do not freeze.
Regulatory StatusResearch Use Only

Scientific Background

CD8a is a 32-34 kD type I glycoprotein. It forms a homodimer (CD8a/a) or heterodimer (CD8a/b) with CD8b. CD8, also known as T8 and Leu2, is a member of the immunoglobulin superfamily found on the majority of thymocytes, a subset of peripheral blood T cells, and NK cells (which express almost exclusively CD8a homodimers). CD8 acts as a co-receptor with MHC class I-restricted T cell receptors in antigen recognition and T cell activation and has been shown to play a role in thymic differentiation. Two domains in CD8a are important for function: the extracellular IgSF domain binds the α3domain of MHC class I and the cytoplasmic CXCP motif binds the tyrosine kinase p56 Lck.

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