Brilliant Violet 711 anti-human CD115 CSF-1R Antibody anti-CD115 - 9-4D2-1E4,BioLegend,347317

It has been reported that CD115 can be rapidly internalized, especially when samples are exposed to room temperature. Approximate 33% decrease in CD115 expression has been observed between 0 and 4 hours after sample collection, while overnight incubation of the cells results in complete CD115 downregulation. Pre-treatment with EDTA and low temperatures (2 to 8°C) helps in maintaining surface expression of CD1151.

Host

Rat

Reactivity

Human

Application

FC -Quality tested

Platform ID

BAB886210261

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Product Specifications
Scientific Background

Specifications

NameBrilliant Violet 711 anti-human CD115 CSF-1R Antibody anti-CD115 - 9-4D2-1E4
Cat. No.347317
HostRat
RRIDAB_2687277 (BioLegend Cat. No. 347317)AB_2687278 (BioLegend Cat. No. 347318)
IsotypeRat IgG1, κ
ReactivityHuman
ApplicationFC -Quality tested
ClonalityMonoclonal
Clone Number9-4D2-1E4
ConcentrationLot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.)
TargetCD115
ImmunogenC-fms transduced Kirsten strain murine sarcoma virus transformed NRK cells.
PurityThe antibody was purified by affinity chromatography and conjugated with Brilliant Violet 711™ under optimal conditions.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
StorageThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light.Do not freeze.
Regulatory StatusResearch Use Only

Scientific Background

CSF-1R, also known as CD115 and M-CSFR, is a single-pass type I membrane protein and member of the platelet-derived growth factor receptor family. Structural studies of CD115 have described an Ig-like extracellular domain, a transmembrane domain, an intracellular juxtamembrane domain, a split tyrosine kinase domain, and a C-terminal tail receptor. Receptor activation induces homodimerization in addition to phosphorylation and ubiquitinylation of intracellular residues. The natural ligands of CD115 include M-CSF and IL-34. CD115 directly influences tissue macrophage and osteoclast differentiation and proliferation. It is expressed on monocytes/macrophages, plasmacytoid and conventional dendritic cells, and osteoclasts.

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