Brilliant Violet 711™ anti-mouse CD24 Antibody, CD24, M1/69,BioLegend,101851

Additional reported applications (for the relevant formats) include: Western blotting1,in vitroinduction of thymocyte maturation2, complement-mediated cytotoxicity3, and immunohistochemistry of acetone-fixed frozen sections4, formalin-fixed paraffin-embedded sections5and zinc-fixed paraffin-embedded sections10. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No.101845 and 101846).

Host

Rat

Reactivity

Mouse

Platform ID

BAB559699475

BioLegend

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Contact

Tel: 1-858-455-9588
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Product Specifications
Scientific Background

Specifications

NameBrilliant Violet 711™ anti-mouse CD24 Antibody, CD24, M1/69
Cat. No.101851
HostRat
RRIDAB_3083100 (BioLegend Cat. No. 101851)
IsotypeRat IgG2b, κ
ReactivityMouse
ClonalityMonoclonal
Clone NumberM1/69
Concentration0.2 mg/mL
TargetCD24
ImmunogenC57BL/10 mouse splenic T cells and concanavalin A-activated splenocytes
PurityThe antibody was purified by affinity chromatography and conjugated with Brilliant Violet 711™ under optimal conditions.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
StorageThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light.Do not freeze.
Regulatory StatusResearch Use Only

Scientific Background

CD24 is a 35-45 kD protein also known as Heat Stable Antigen (HSA), Ly-52, or Nectadrin. It is a GPI-linked sialoglycoprotein expressed on lymphocytes, granulocytes, epithelial cells, thymocytes, monocytes, erythrocytes, and dendritic cells. CD24 expression varies during T and B cell differentiation and is a useful marker for delineating various lymphocyte developmental stages. CD24 serves as an adhesion or costimulatory molecule involved in T and B lymphocyte activation and differentiation by homophilic binding or binding to CD62P.

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