Brilliant Violet 785 anti-mouse CD45.1 Antibody anti-CD45.1 - A20,BioLegend,110743

The A20 antibody does not react with leukocytes or mouse cells expressing the CD45.2 alloantigen. Additional reported applications (for relevant formats of this clone) include: immunoprecipitation3,in vitroblocking of B cell responses1,2, immunohistochemical staining of frozen sections: OCT embedded7and acetone-fixed4-6(direct immunofluorescence detection with fluorochrome conjugated A20 was used in (5) and (6)).

Host

Mouse

Reactivity

Mouse

Application

FC - Quality tested

Platform ID

BAB202514684

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Product Specifications
Scientific Background

Specifications

NameBrilliant Violet 785 anti-mouse CD45.1 Antibody anti-CD45.1 - A20
Cat. No.110743
HostMouse
RRIDAB_2563379 (BioLegend Cat. No. 110743)
IsotypeMouse (A.SW) IgG2a, κ
ReactivityMouse
ApplicationFC - Quality tested
ClonalityMonoclonal
Clone NumberA20
Concentration0.2 mg/ml
TargetCD45.1
ImmunogenSJL mouse thymocytes and splenocytes
PurityThe antibody was purified by affinity chromatography and conjugated with Brilliant Violet 785™ under optimal conditions.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
StorageThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light.Do not freeze.
Regulatory StatusResearch Use Only

Scientific Background

CD45.1 is an alloantigen of CD45, expressed by Ly5.1 bearing mouse strains (e.g., RIII, SJL/J, STS/A, DA). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in mice that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell types. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

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